JoVE Logo

登录

Universidade de Sao Paulo

5 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Positron Emission Tomography Imaging for In Vivo Measuring of Myelin Content in the Lysolecithin Rat Model of Multiple Sclerosis
Daniele de Paula Faria 1, Caroline Cristiano Real 1, Larissa Estessi de Souza 1, Alexandre Teles Garcez 2, Fabio Luis Navarro Marques 1, Carlos Alberto Buchpiguel 1
1Laboratory of Nuclear Medicine (LIM-43), Departamento de Radiologia e Oncologia, Faculdade de Medicina, Universidade de Sao Paulo, 2Nuclear Medicine Division, Hospital Sirio-Libanes

This protocol has the aim of monitoring in vivo myelin changes (demyelination and remyelination) by positron emission tomography (PET) imaging in an animal model of multiple sclerosis.

image

Neuroscience

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
Josiane do Nascimento Silva 1, Thais T. Zampieri 1, Henrique R. Vieira 1, Renata Frazao 1
1Department of Anatomy, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo

Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.

image

Medicine

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
Thaís Girão-Silva *1,2, Miriam Helena Fonseca-Alaniz *1, Luís Alberto Oliveira Dallan 1, Iuri Cordeiro Valãdao 1, Gustavo Henrique Oliveira da Rocha 1, José Eduardo Krieger 1, Ayumi Aurea Miyakawa 1
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg

We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in hSVECs.

image

Medicine

Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
Paolo Oliveira *1,3, Keiji Yamanashi *1, Aizhou Wang 1, Marcelo Cypel 1,2
1Toronto General Hospital Research Institute, University Health Network, 2Department of Surgery, University of Toronto, 3Departamento de Cardiopneumologia, Instituto do Coração, Faculdade de Medicina HCFMUSP, Universidade de Sao Paulo

Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.

image

Medicine

Monitoring Lung Function with Electrical Impedance Tomography in the Intensive Care Unit
Glasiele C. Alcala *1, Gamonmas Ekkapat *1,2, Kyle J. Medeiros 1, Caio C. A Morais 1, Yi Xin 1, Valentina Giammatteo 1,3, Giovanni Bruno 1, Alice Nova 1,4, Hatus Wanderley 1, Talisa Bühl 1, Marcus Victor 1,6, Timothy G. Gaulton 1, Carolyn J. La Vita 5, Marcelo B. P. Amato 7, Maurizio Ceradda 1, Lorenzo Berra 1, Roberta Ribeiro De Santis Santiago 1
1Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, 2Department of Anesthesiology, Faculty of Medicine, Chulalongkorn University. King Chulalongkorn Memorial Hospital, 3Department of Anesthesiology and Intensive Care Medicine, Catholic University of Sacred Heart, 4School of Medicine and Surgery, University of Milano-Bicocca, 5Department of Respiratory Care, Massachusetts General Hospital, Harvard Medical School, 6Electronics Engineering, Aeronautics Institute of Technology, 7Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo

Electrical Impedance Tomography is a non-invasive, radiation-free, real-time pulmonary ventilation monitoring tool. By measuring impedance changes in the thorax, it can visualize the distribution of air on a breath-by-breath basis. Initially intended for ventilation monitoring, electrical impedance tomography can also measure perfusion via intravenous injection of a saline solution.

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。