登录

del Centro de Investigación y de Estudios Avanzados (CINVESTAV)

3 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
Abigail Betanzos 1, Michael Schnoor 2, Rosario Javier-Reyna 1, Guillermina García-Rivera 1, Cecilia Bañuelos 3, Jonnatan Pais-Morales 1, Esther Orozco 1
1Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Agency for Knowledge Commercialization, Center for Research and Advanced Studies of the National Polytechnic Institute

Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.

image

Medicine

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Hilda Vargas Robles 1, Karla Fabiola Castro Ochoa 1, Porfirio Nava 2, Angélica Silva Olivares 3, Mineko Shibayama 3, Michael Schnoor 1
1Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute

Current treatments of inflammatory bowel diseases (IBD) aim at alleviating disease symptoms but may cause severe side effects. Thus, alternative strategies are being investigated in animal colitis models. Here, we explain how the beneficial effects of diet supplements on clinical IBD signs are analyzed in such a colitis model.

image

Biology

Evaluation and Quantification of Micro Epithelial Gaps in the Colonic Mucosa using Immunofluorescence Staining
Felipe Castro-Martínez *1, María del Rocío Encarnación-García *1, Aurora Candelario-Martinez *1, Oscar Medina-Contreras 3, Genaro Patiño-Lopez 4, Michael Schnoor 2, Porfirio Nava 1
1Departamento de Fisiología, Biofísica y Neurociencias del Centro de Investigación y de Estudios Avanzados (CINVESTAV), 2Departamento de Biomedicina Molecular, del Centro de Investigación y de Estudios Avanzados (CINVESTAV), 3Unidad de Investigación Epidemiológica en Endocrinología, y Nutrición (UIEEN), Hospital Infantil de México Federico Gomez, 4Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez

Here, we describe a new method to visualize the specific location of where transcellular and paracellular permeability is enhanced in the inflamed colonic mucosa. In this assay, we apply a 10 kDa fluorescent dye conjugated to a lysine fixable dextran to visualize high permeability regions (HPR) in the colonic mucosa.

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。