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del Centro de Investigación y de Estudios Avanzados (CINVESTAV)

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Abigail Betanzos¹, Michael Schnoor², Rosario Javier-Reyna¹, Guillermina García-Rivera¹, Cecilia Bañuelos³, Jonnatan Pais-Morales¹, Esther Orozco¹

¹Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, ²Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, ³Agency for Knowledge Commercialization, Center for Research and Advanced Studies of the National Polytechnic Institute

Human infection by Entamoeba histolytica leads to amoebiasis, a major cause of diarrhea in tropical countries. Infection is initiated by pathogen interactions with intestinal epithelial cells, provoking the opening of cell-cell contacts and consequently diarrhea, sometimes followed by liver infection. This article provides a model to assess the early host-pathogen interactions to improve our understanding of amoebiasis pathogenesis.

JoVE Core

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Hilda Vargas Robles¹, Karla Fabiola Castro Ochoa¹, Porfirio Nava², Angélica Silva Olivares³, Mineko Shibayama³, Michael Schnoor¹

¹Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, ²Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, ³Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute

Current treatments of inflammatory bowel diseases (IBD) aim at alleviating disease symptoms but may cause severe side effects. Thus, alternative strategies are being investigated in animal colitis models. Here, we explain how the beneficial effects of diet supplements on clinical IBD signs are analyzed in such a colitis model.

Biology

Evaluation and Quantification of Micro Epithelial Gaps in the Colonic Mucosa using Immunofluorescence Staining

Felipe Castro-Martínez^*1, María del Rocío Encarnación-García^*1, Aurora Candelario-Martinez^*1, Oscar Medina-Contreras³, Genaro Patiño-Lopez⁴, Michael Schnoor², Porfirio Nava¹

¹Departamento de Fisiología, Biofísica y Neurociencias del Centro de Investigación y de Estudios Avanzados (CINVESTAV), ²Departamento de Biomedicina Molecular, del Centro de Investigación y de Estudios Avanzados (CINVESTAV), ³Unidad de Investigación Epidemiológica en Endocrinología, y Nutrición (UIEEN), Hospital Infantil de México Federico Gomez, ⁴Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez

Here, we describe a new method to visualize the specific location of where transcellular and paracellular permeability is enhanced in the inflamed colonic mucosa. In this assay, we apply a 10 kDa fluorescent dye conjugated to a lysine fixable dextran to visualize high permeability regions (HPR) in the colonic mucosa.