Sanofi, Framingham, Massachusetts
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
Christina M. Bailey-Hytholt *1, Paroma Ghosh *1, Julia Dugas 1, Isidro E. Zarraga 1, Amey Bandekar 1
1Biologics Drug Product Development & Manufacturing, Sanofi, Framingham, Massachusetts
Lipid nanoparticles are developed using a microfluidic mixing platform approach for mRNA and DNA encapsulation.
Bioengineering
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Christina M. Bailey-Hytholt 1, Veronica LaMastro 2, Anita Shukla 2
1Department of Chemical Engineering, Worcester Polytechnic Institute, 2Center for Biomedical Engineering, School of Engineering, Brown University
This protocol describes the formation of cell mimicking uni-lipid and multi-lipid vesicles, supported lipid bilayers, and suspended lipid bilayers. These in vitro models can be adapted to incorporate a variety of lipid types and can be used to investigate various molecule and macromolecule interactions.
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