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Sanofi, Framingham, Massachusetts

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Christina M. Bailey-Hytholt^*1, Paroma Ghosh^*1, Julia Dugas¹, Isidro E. Zarraga¹, Amey Bandekar¹

¹Biologics Drug Product Development & Manufacturing, Sanofi, Framingham, Massachusetts

Lipid nanoparticles are developed using a microfluidic mixing platform approach for mRNA and DNA encapsulation.

Bioengineering

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Christina M. Bailey-Hytholt¹, Veronica LaMastro², Anita Shukla²

¹Department of Chemical Engineering, Worcester Polytechnic Institute, ²Center for Biomedical Engineering, School of Engineering, Brown University

This protocol describes the formation of cell mimicking uni-lipid and multi-lipid vesicles, supported lipid bilayers, and suspended lipid bilayers. These in vitro models can be adapted to incorporate a variety of lipid types and can be used to investigate various molecule and macromolecule interactions.