Leibniz Institute on Aging, Fritz-Lipmann-Institute
2 ARTICLES PUBLISHED IN JoVE
Genetics
Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
Hellen E. Ahrens *1, Henriette Henze *1, Svenja C. Schüler 1, Manuel Schmidt 1, Sören S. Hüttner 1, Julia von Maltzahn 1
1Leibniz-Institute on Aging - Fritz-Lipmann-Institute
We describe an in vivo method to investigate the functional loss of a specific gene in satellite cells by using a combination of cardiotoxin mediated injury of skeletal muscle and the injection of a self-delivering siRNA.
Biology
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Sören S. Hüttner 1, Christian Hayn 1, Hellen E. Ahrens 1, Manuel Schmidt 1, Henriette Henze 1, Julia von Maltzahn 1
1Leibniz Institute on Aging, Fritz-Lipmann-Institute
In this protocol an in vitro culturing and functional analysis method for muscle stem cells is described, which preserves most of their interactions with their endogenous niche.
