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Leibniz Institute on Aging, Fritz-Lipmann-Institute

2 ARTICLES PUBLISHED IN JoVE

Genetics

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Hellen E. Ahrens^*1, Henriette Henze^*1, Svenja C. Schüler¹, Manuel Schmidt¹, Sören S. Hüttner¹, Julia von Maltzahn¹

¹Leibniz-Institute on Aging - Fritz-Lipmann-Institute

We describe an in vivo method to investigate the functional loss of a specific gene in satellite cells by using a combination of cardiotoxin mediated injury of skeletal muscle and the injection of a self-delivering siRNA.

Biology

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Sören S. Hüttner¹, Christian Hayn¹, Hellen E. Ahrens¹, Manuel Schmidt¹, Henriette Henze¹, Julia von Maltzahn¹

¹Leibniz Institute on Aging, Fritz-Lipmann-Institute

In this protocol an in vitro culturing and functional analysis method for muscle stem cells is described, which preserves most of their interactions with their endogenous niche.