Diamond Light Source Ltd, Harwell Science and Innovation Campus

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Adam D. Crawshaw¹, Emma V. Beale^1,2, Anna J. Warren¹, Andrew Stallwood^1,3, Graham Duller¹, Jose Trincao¹, Gwyndaf Evans^1,4

¹Diamond Light Source Ltd, Harwell Science and Innovation Campus, ²Paul Scherrer Institut, ³Central Laser Facility, Science and Technologies Facilities Council, Harwell Science and Innovation Campus, ⁴Rosalind Franklin Institute, Harwell Science and Innovation Campus

The signal-to-noise ratio of data is one of the most important considerations in performing X-ray diffraction measurements from microcrystals. The VMXm beamline provides a low-noise environment and microbeam for such experiments. Here, we describe sample preparation methods for mounting and cooling microcrystals for VMXm and other microfocus macromolecular crystallography beamlines.

Chemistry

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Alice Douangamath^*1,2, Ailsa Powell^*1,2, Daren Fearon^*1,2, Patrick M. Collins^1,2, Romain Talon^1,2,3, Tobias Krojer^3,4, Rachael Skyner^1,2, Jose Brandao-Neto^1,2, Louise Dunnett^1,2, Alexandre Dias^1,2, Anthony Aimon^1,2,3, Nicholas M. Pearce^1,3, Conor Wild^3,5, Tyler Gorrie-Stone¹, Frank von Delft^1,2,3,4,6

¹Diamond Light Source Ltd, Harwell Science and Innovation Campus, ²Research Complex at Harwell, Harwell Science and Innovation Campus, ³Structural Genomics Consortium, University of Oxford, ⁴Centre for Medicines Discovery, University of Oxford, ⁵Oxford Protein Informatics Group, Department of Statistics, Oxford University, ⁶Department of Biochemistry, University of Johannesburg

This paper describes the complete XChem process for crystal-based fragment screening, starting from applying for access and all subsequent steps to data dissemination.