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Harwell Science and Innovation Campus

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
Adam D. Crawshaw 1, Emma V. Beale 1,2, Anna J. Warren 1, Andrew Stallwood 1,3, Graham Duller 1, Jose Trincao 1, Gwyndaf Evans 1,4
1Diamond Light Source Ltd, Harwell Science and Innovation Campus, 2Paul Scherrer Institut, 3Central Laser Facility, Science and Technologies Facilities Council, Harwell Science and Innovation Campus, 4Rosalind Franklin Institute, Harwell Science and Innovation Campus

The signal-to-noise ratio of data is one of the most important considerations in performing X-ray diffraction measurements from microcrystals. The VMXm beamline provides a low-noise environment and microbeam for such experiments. Here, we describe sample preparation methods for mounting and cooling microcrystals for VMXm and other microfocus macromolecular crystallography beamlines.

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Biochemistry

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source
James Sandy 1, Halina Mikolajek 1,2, Amy J. Thompson 1, Juan Sanchez-Weatherby 1, Michael A Hough 1,2
1Diamond Light Source Ltd, Harwell Science and Innovation Campus, 2Research Complex at Harwell, Harwell Science and Innovation Campus

We present a protocol for the crystallization of proteins using the crystallization facility in the Research Complex at Harwell and subsequent in situ X-ray crystallographic data collection from crystals within the plates at Diamond's Versatile Macromolecular Crystallography in situ (VMXi) beamline. We describe sample requirements, crystallization protocols, and data collection guidelines.

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