Christian Medical College Campus
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells
Gokulnath Mahalingam *1, Aruna Mohan *1, Porkizhi Arjunan *1, Ajay Kumar Dhyani 1, Kanimozhi Subramaniyam 1, Yogapriya Periyasamy 1, Srujan Marepally 1
1Centre for Stem Cell Research, Christian Medical College Campus
The protocol presents in vitro transcription (IVT) of chemically modified mRNA, cationic liposome preparation, and functional analysis of liposome enabled mRNA transfections in mammalian cells.
Biology
CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
Vigneshwaran Venkatesan *1,2, Abisha Crystal Christopher *1,3, Karthik V. Karuppusamy 1,2, Prathibha Babu 1,2, Manoj Kumar K. Alagiri 1,2, Saravanabhavan Thangavel 1
1Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru, Christian Medical College campus, 2Manipal Academy of Higher Education, 3Thiruvalluvar University
The present protocol describes an optimized hematopoietic stem and progenitor cell (HSPC) culture procedure for the robust engraftment of gene-edited cells in vivo.
