Accedi

Christian Medical College Campus

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Gokulnath Mahalingam^*1, Aruna Mohan^*1, Porkizhi Arjunan^*1, Ajay Kumar Dhyani¹, Kanimozhi Subramaniyam¹, Yogapriya Periyasamy¹, Srujan Marepally¹

¹Centre for Stem Cell Research, Christian Medical College Campus

The protocol presents in vitro transcription (IVT) of chemically modified mRNA, cationic liposome preparation, and functional analysis of liposome enabled mRNA transfections in mammalian cells.

Biology

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Vigneshwaran Venkatesan^*1,2, Abisha Crystal Christopher^*1,3, Karthik V. Karuppusamy^1,2, Prathibha Babu^1,2, Manoj Kumar K. Alagiri^1,2, Saravanabhavan Thangavel¹

¹Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru, Christian Medical College campus, ²Manipal Academy of Higher Education, ³Thiruvalluvar University

The present protocol describes an optimized hematopoietic stem and progenitor cell (HSPC) culture procedure for the robust engraftment of gene-edited cells in vivo.