European Institute of Oncology IRCCS (IEO)
2 ARTICLES PUBLISHED IN JoVE
Biology
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
Monica Soldi 1, Tiziana Bonaldi 1
1Department of Experimental Oncology, European Institute of Oncology
By combining native and crosslinking chromatin immunoprecipitation with high-resolution Mass Spectrometry, ChroP approach enables to dissect the composite proteomic architecture of histone modifications, variants and non-histonic proteins synergizing at functionally distinct chromatin domains.
Biochemistry
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Marianna Maniaci *1,2, Federica Marini *1, Enrico Massignani 1,2, Tiziana Bonaldi 1
1Department of Experimental Oncology, European Institute of Oncology IRCCS (IEO), 2European School of Molecular Medicine (SEMM), c/o Campus IFOM-IEO
Protein Arginine (R)-methylation is a wide-spread post-translational modification regulating multiple biological pathways. Mass spectrometry is the best technology to globally profile the R-methyl-proteome, when coupled to biochemical approaches for modified peptide enrichment. The workflow designed for the high confidence identification of global R-methylation in human cells is described here.
