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European Institute of Oncology IRCCS (IEO)

2 ARTICLES PUBLISHED IN JoVE

Biology

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

¹Department of Experimental Oncology, European Institute of Oncology

By combining native and crosslinking chromatin immunoprecipitation with high-resolution Mass Spectrometry, ChroP approach enables to dissect the composite proteomic architecture of histone modifications, variants and non-histonic proteins synergizing at functionally distinct chromatin domains.

Biochemistry

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Marianna Maniaci^*1,2, Federica Marini^*1, Enrico Massignani^1,2, Tiziana Bonaldi¹

¹Department of Experimental Oncology, European Institute of Oncology IRCCS (IEO), ²European School of Molecular Medicine (SEMM), c/o Campus IFOM-IEO

Protein Arginine (R)-methylation is a wide-spread post-translational modification regulating multiple biological pathways. Mass spectrometry is the best technology to globally profile the R-methyl-proteome, when coupled to biochemical approaches for modified peptide enrichment. The workflow designed for the high confidence identification of global R-methylation in human cells is described here.