CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
Florence A. Giger 1, Julien G. Dumortier 2, Nicolas B. David 1
1CNRS UMR8197 – INSERM U1024, IBENS, Institut de Biologie de l'École Normale Supérieure, 2Department of Physiology Development and Neuroscience, University of Cambridge
Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.
Developmental Biology
Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula
Arthur Boutillon 1, Sophie Escot 1, Nicolas B. David 1
1Laboratory for Optics and Biosciences, CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris
Embryonic development requires large-scale coordination of cell motion. Two-photon excitation mediated laser ablation allows the spatially controlled 3-dimensional ablation of large groups of deep cells. In addition, this technique can probe the reaction of collectively migrating cells in vivo to perturbations in their mechanical environment.
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