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CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Florence A. Giger¹, Julien G. Dumortier², Nicolas B. David¹

¹CNRS UMR8197 – INSERM U1024, IBENS, Institut de Biologie de l'École Normale Supérieure, ²Department of Physiology Development and Neuroscience, University of Cambridge

Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.

Developmental Biology

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Arthur Boutillon¹, Sophie Escot¹, Nicolas B. David¹

¹Laboratory for Optics and Biosciences, CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris

Embryonic development requires large-scale coordination of cell motion. Two-photon excitation mediated laser ablation allows the spatially controlled 3-dimensional ablation of large groups of deep cells. In addition, this technique can probe the reaction of collectively migrating cells in vivo to perturbations in their mechanical environment.