Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center

1 ARTICLES PUBLISHED IN JoVE

Biology

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Alan Y. Hsu¹, Zhicheng Peng¹, Hongbo Luo¹, Fabien Loison^1,2

¹Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, ²Department of Microbiology, Faculty of Science, Mahidol University

This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.