Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular SpheroidsIrina A. Okkelman *1, Chris Vercruysse *1, Alina V. Kondrashina 2, Sergey M. Borisov 3, Ruslan I. Dmitriev 1
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, 2Health and Happiness (H&H) Group, National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology
The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.
