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Ulsan National Institute of Science and Technology

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
Yujin Kang 1, Subin Bae 1, Soyeong An 1, Ja Yil Lee 1,2
1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS)

DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.

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