Ulsan National Institute of Science and Technology

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Yujin Kang¹, Subin Bae¹, Soyeong An¹, Ja Yil Lee^1,2

¹Department of Biological Sciences, Ulsan National Institute of Science and Technology, ²Center for Genomic Integrity, Institute for Basic Science (IBS)

DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.