Preparation of Rat Sciatic Nerve for Ex Vivo NeurophysiologyAdrien Rapeaux 1,2,3,4, Omaer Syed 5, Estelle Cuttaz 5, Christopher A. R. Chapman 5, Rylie A. Green 5, Timothy G. Constandinou 1,2,3,4
1Department of Electrical and Electronic Engineering, Imperial College London, 2Centre for Bioinspired Technology, Imperial College London, 3Care Research and Technology (CR&T), Imperial College London and the University of Surrey, 4Dementia Institute (UK DRI), 5Department of Bioengineering, Imperial College London
This protocol describes the preparation of rat whole sciatic nerve tissue for ex vivo electrophysiological stimulation and recording in an environmentally-regulated, two-compartment, perfused saline bath.
