登录

University of North Carolina at Chapel Hill and North Carolina State University

1 ARTICLES PUBLISHED IN JoVE

image

Bioengineering

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
Madelyn VanBlunk 1, Pritha Agarwalla 1,2, Sharda Pandit 1,2, Yevgeny Brudno 1,2,3
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute, North Carolina State University, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。