San Diego Zoo Wildlife Alliance

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Laura Adams^*1, Ying Liu^*1, Barbara Durrant², Elena Ruggeri², Carly Young², Rebecca Krisher³, Irina Polejaeva¹

¹Department of Animal, Dairy, and Veterinary Sciences, Utah State University, ²Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, ³Genus PLC

Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.