Results: Successful and Unsuccessful qPCR Results and Boxplot from Mitochondria-Reduced and Mitochondria-Dense Ooplasts
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Conclusion
副本
The protocol significantly reduces the mitochondrial DNA copy number in the oocyte. That might be beneficial for intraspecies cloning. This method reduces mitochondrial DNA copy numbers in bovine oocytes by over 90%which could reduce the incidence
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Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
