Toronto General Hospital Research Institute, University Health Network
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
Michael S. Xu 1, Golnaz Karoubi 1,2, Thomas K. Waddell 1,3, Siba Haykal 1,4
1Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, 2Institute of Laboratory Medicine and Pathobiology, University of Toronto, 3Division of Thoracic Surgery, Department of Surgery, University of Toronto, 4Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Toronto
The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.
Bioengineering
Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation
Aisha Adil 1,2, Golnaz Karoubi 1,3, Siba Haykal 1,2,4
1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto
We describe the surgical technique and decellularization process for composite rat hindlimbs. Decellularization is conducted using low-concentration sodium dodecyl sulfate through an ex vivo machine perfusion system.
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