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Toronto General Hospital Research Institute, University Health Network

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Michael S. Xu¹, Golnaz Karoubi^1,2, Thomas K. Waddell^1,3, Siba Haykal^1,4

¹Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, ²Institute of Laboratory Medicine and Pathobiology, University of Toronto, ³Division of Thoracic Surgery, Department of Surgery, University of Toronto, ⁴Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Toronto

The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.

Bioengineering

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Aisha Adil^1,2, Golnaz Karoubi^1,3, Siba Haykal^1,2,4

¹Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, ²Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, ³Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, ⁴Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto

We describe the surgical technique and decellularization process for composite rat hindlimbs. Decellularization is conducted using low-concentration sodium dodecyl sulfate through an ex vivo machine perfusion system.