Bucknell University
3 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Micromanipulation of Chromosomes in Insect Spermatocytes
Nicolas K.H. Lin 1, Ryder Nance 2, Jane Szybist 2, Alan Cheville 2, Leocadia V. Paliulis 3
1Program in Cell Biology/Biochemistry, Bucknell University, 2Department of Electrical and Computer Engineering, Bucknell University, 3Biology Department, Bucknell University
In this protocol, we describe the selection and preparation of appropriate cells for micromanipulation and the use of a piezoelectric micromanipulator to reposition chromosomes within those cells.
Chemistry
Efficient Synthesis of All-Carbon Quaternary Centers via the Conjugate Addition of Functionalized Monoorganozinc Bromides
Tyler J. Fulton 1, Katherine M. Townsend 2, Michael R. Krout 2
1Warren and Katharine Schlinger Laboratory for Chemistry and Chemical Engineering, California Institute of Technology, 2Department of Chemistry, Bucknell University
A simple and practical protocol for the efficient conjugate addition of functionalized monoorganozinc bromides to cyclic α,β-unsaturated carbonyls to furnish all-carbon quaternary centers was developed.
Biochemistry
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Rebecca Switzer 1, Katie A. Ward 2, Jessica Medrano 2
1Department of Chemistry, Bucknell University, 2Program in Cell Biology/Biochemistry, Bucknell University
DNA methyltransferases are potential cancer drug targets. Here, a protocol is presented to assess small molecules for DNA methyltransferase inhibition. This assay utilizes an endonuclease to couple DNA methylation to fluorescence generation and allows for enzyme activity to be monitored in real time.
