Preparation of Primary Insect Spermatocyte Cell Culture for Micromanipulation
1:11
Spermatocyte Culture Preparation for Viewing on Microscope
2:03
Production of the Microneedle
2:42
Positioning the Micromanipulator
4:00
Micromanipulation of Chromosomes
4:43
Results: Chromosomes Can be Detached from Spindle in Prophase, Metaphase, and Anaphase
5:07
Conclusion
副本
This method can help answer key questions in cell biology, such as, how important is tension in satisfying the spindle checkpoint, and what is the role on spindle attachment in chromosome behavior in miosis? The main advantage of this technique is
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In this protocol, we describe the selection and preparation of appropriate cells for micromanipulation and the use of a piezoelectric micromanipulator to reposition chromosomes within those cells.
