Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived SamplesRoberto Plebani 1,2, Alessandra D'Alessandro 2, Paola Lanuti 2,3, Pasquale Simeone 2,3, Massimiliano Cinalli 1, Ilaria Righi 4, Alessandro Palleschi 4,5, Matteo Mucci 1,2, Marco Marchisio 2,3, Francesco Cappabianca 1, Marina Camera 6,7, Felice Mucilli 1, Mario Romano 1,2
1Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences, University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone, Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Università degli Studi di Milano, 6Department of Pharmaceutical Sciences, Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases, Centro Cardiologico Monzino IRCCS
Although challenging, the isolation of pulmonary endothelial cells is essential for studies on lung inflammation. The present protocol describes a procedure for the high-yield, high-purity isolation of macrovascular and microvascular endothelial cells.
