Waters Corporation
2 ARTICLES PUBLISHED IN JoVE
Basic Principles for Purification Using Supercritical Fluid Chromatography - ADVERTISEMENT
Jo-Ann M. Jablonski 1, Christopher J. Hudalla 1, Kenneth J. Fountain 1, Steven M. Collier 1, Damian Morrison 1
1Chemistry Commercial Operations, Waters Corporation
Basic principles for the separation of compounds from mixtures using supercritical fluid chromatography (SFC) are similar to the fundamentals of preparative liquid chromatography.
Chemistry
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
Catalin Doneanu *1, Jing Fang *1, Yun Alelyunas *1, Ying Qing Yu 1, Mark Wrona 1, Weibin Chen 1
1Waters Corporation
Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.
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