Waters Corporation

Basic principles for the separation of compounds from mixtures using supercritical fluid chromatography (SFC) are similar to the fundamentals of preparative liquid chromatography.

Here, we describe a chromatographic assay coupled with the ion mobility separation of peptide precursors followed by the high-resolution (~30,000) MS-detection of peptide fragments for the quantification of spiked peptide standards in a monoclonal antibody digest.