Justus-Liebig-Universität Gießen

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Sudhanshu Bhushan¹, Ferial Aslani¹, Zhengguo Zhang¹, Tim Sebastian¹, Hans-Peter Elsässer², Jörg Klug¹

¹Institut für Anatomie und Zellbiologie, Justus-Liebig-Universität Gießen, ²Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg

Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.