Isolation of Sertoli Cells and Peritubular Cells from Rat TestesSudhanshu Bhushan 1, Ferial Aslani 1, Zhengguo Zhang 1, Tim Sebastian 1, Hans-Peter Elsässer 2, Jörg Klug 1
1Institut für Anatomie und Zellbiologie, Justus-Liebig-Universität Gießen, 2Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg
Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.
