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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

DOI :

10.3791/1622-v

December 2nd, 2009

December 2nd, 2009

11,605 Views

1Department of Molecular and Cellular Biology, Brown University, 2Center for Computational Molecular Biology, Brown University

Due to the transient nature of pre-mRNA, it can be difficult to isolate and study in vivo. Here, we present a novel in vitro approach to investigate RNA-protein interactions using a synthetic oligo pool that tiles across selected regions of pre-mRNA.

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Rapid High throughput Method

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