The video demonstrates how to set up a counterbalance system for a microdrive implanted in a mouse. It involves connecting a PVC pipe, positioning arms above the cage, attaching the microdrive with a corresponding weight, and ensuring proper access for the mouse while securing the setup to the electronic interface board.

recovery after micro-drive implantation

用于记录幼年小鼠日常神经活动的 Micro-drive 植入物

The brain undergoes large-scale changes during the critical developmental windows of childhood and adolescence1,2,3. Many neurologic and psychiatric diseases, including autism and schizophrenia, first manifest behaviorally and biologically during this period of juvenile and adolescent brain development4,5,6. While much is known regarding the cellular, synaptic, and genetic changes that occur across early development, comparatively little is known regarding how circuit- or network-level processes change throughout this time window. Importantly, circuit-level brain function, which ultimately underlies complex behaviors, memory, and cognition, is a non-predictable, emergent property of cellular and synaptic function7,8,9,10. Thus, to fully understand network-level brain function, it is necessary to directly study neural activity at the level of an intact neural circuit. In addition, to identify how brain activity is altered throughout the progression of neuropsychiatric disorders, it is critical to examine network activity in a valid disease model during the specific temporal window when the behavioral phenotypes of the disease manifest and to track the observed changes as they persist into adulthood.
One of the most common and powerful scientific model organisms is the mouse, with large numbers of unique genetic strains that model neurodevelopmental disorders with age-dependent onset of the behavioral and/or mnemonic phenotypes11,12,13,14,15,16,17,18,19,20,21. While it is challenging to correlate precise developmental time points between the brains of humans and mice, morphological and behavioral comparisons indicate that p20-p21 mice represent the human ages of 2-3 years old, and p25-p35 mice represent the human ages of 11-14 years old, with mice likely reaching the developmental equivalent of a human 20 year old adult by p603,22. Thus, to better understand how the juvenile brain develops and to identify how the neural networks of the brain become dysfunctional in diseases like autism or schizophrenia, it would be ideal to directly monitor brain activity in vivo in mice across the ages of 20 days to 60 days old.
However, a fundamental challenge in monitoring brain activity across early development in mice is the small size and relative weakness of juvenile mice. The chronic implantation of electrodes, which is necessary for longitudinal studies of brain development, typically requires large, bulky housing to protect the fine electrode wires and interface boards23,24, and the implants must be firmly attached to the mouse skull, which is thinner and less rigid in young mice due to reduced ossification. Thus, virtually all studies of in vivo rodent physiology have been performed in adult subjects due to their relative size, strength, and skull thickness. To date, most studies exploring in vivo juvenile rodent brain physiology have been performed in wild-type juvenile rats, which necessarily limits the ability to experimentally monitor juvenile brain function in a freely behaving model of a human disorder25,26,27,28,29,30.
This manuscript describes novel implant housing, a surgical implantation procedure, and a post-surgery recovery strategy to chronically study the long-term (up to 4 or more weeks) in vivo brain function of juvenile mice across a developmentally critical time window (p20 to p60 and beyond). The implantation procedure allows for the reliable, permanent affixation of the electrodes to the skulls of juvenile mice. Furthermore, the micro-drive design is lightweight, as this micro-drive weighs ~4-6 g when fully assembled, and due to the minimal counterbalancing required to offset the weight of the implant, it does not impact the behavioral performance of juvenile mice during typical behavioral paradigms.

作者聚焦：用于幼年小鼠慢性四极管记录的轻型驱动植入物  

一种用于幼年小鼠慢性四极管记录的轻型驱动植入物

Watch this Scientific Journal Video about Recovery after Micro-Drive Implantation at JoVE.com

Recovery after Micro-Drive Implantation  

微驱动器植入后的恢复

首先，通过连接直径为 0.75 英寸的 PVC 管来设置平衡系统。将系统的一个臂插入笼盖上的孔中。将第二只臂放在笼盖的顶部，并将第三只臂伸出笼子上方和之外。最后盖住最上面的手臂。将微型驱动器植入鼠标目标后，使用等于微型驱动器和骨螺钉重量的平衡重量将微型驱动器连接到平衡系统。将一根坚固的线或钓鱼线从电子接口板固定到悬挂在最顶部手臂上的平衡重上。最后，确保平衡牢固地连接到微驱动器电子接口板，并且鼠标可以完全进入笼子。

Research

JoVE Journal

Neuroscience

Recovery after Micro-Drive Implantation

325 次观看. 首先，通过连接直径为 0.75 英寸的 PVC 管来设置平衡系统。将系统的一个臂插入笼盖上的孔中。将第二只臂放在笼盖的顶部，并将第三只臂伸出笼子上方和之外。最后盖住最上面的手臂。将微型驱动器植入鼠标目标后，使用等于微型驱动器和骨螺钉重量的平衡重量将微型驱动器连接到平衡系统。将一根坚固的线或钓鱼线从电子接口板固定到悬挂在最顶部手臂上的平衡重?...

视频: Recovery after Micro-Drive Implantation  

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

In vivo electrophysiology provides unparalleled insight into the sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuropsychiatric disorders. However, such methods often require large cranial implants, which cannot be used in mice at early developmental time points. As such, virtually no studies of in vivo physiology have been performed in freely behaving infant or juvenile mice, despite the fact that a better understanding of neurological development in this critical window would likely provide unique insights into age-dependent developmental disorders such as autism or schizophrenia. Here, a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy are described that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in mice as they age from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond, a time window roughly corresponding to the human ages of 2 years old through to adulthood. The number of recording electrodes and final recording sites can be easily modified and expanded, thus allowing flexible experimental control of the in vivo monitoring of behavior- or disease-relevant brain regions across development.

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.