Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

To begin, take 293FT cells cultured in a 10 centimeter dish, and aspirate the media. Wash cells with five milliliters of PBS. Then add one milliliter of trypsin EDTA, and incubate for 3-5 minutes at 37 degrees Celsius to detach cells from the dish