Name: induction and clinical scoring of chronic-relapsing experimental autoimmune encephalomyelitis
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis at JoVE.com

<p class="jove_title">Induction and monitoring of chronic-relapsing EAE</p>
<p class="jove_content">The emulsion is a 1:1 mixture of antigen in aqueous solution added to supplemented complete Freund's adjuvant. It is very important to add the antigen to the adjuvant and not the other way round!!!</p>
<p class="jove_content">There is a lot of waste when making and injecting an emulsion. Always prepare 1.5 or 2 times more than what you need.</p>
<p class="jove_content"><span class="jove_content">Use autoclaved mortar, pestle, glass syringes, and ...

<p class="jove_content">There are a few important considerations for this protocol.</p>
<p class="jove_content">Genetics of DA rats will differ slightly depending on which breeder they are bought from. These differences may increase or decrease the susceptibility of rats to EAE induction. If your rats are more susceptible to EAE you will want to reduce the strength of the immunization by using non-supplemented complete Freund's adjuvant or even incomplete Freund's adjuvant. You may also consider reducing the amount of emulsion injected or dilu...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Complete Freund's Adjuvant</td>
<td>Reagent</td>
<td>Fisher</td>
<td>DF 311-360-5</td>
<td>Manufactured by Difco</td>
</tr>
<tr>
<td>Mycobacterium tuberculosis H37Ra</td>
<td>Reagent</td>
<td>Fisher</td>
<td>DF3114-33-8</td>
<td>Manufactured by Difco</td>
</tr>
<tr>
<td>DA rat</td>
<td>Animal</td>
<td>Harlan Sprague Dawley</td>
<td>&nbsp;</td>
<td>Females, 7-9 weeks old.</td>
</tr>
<tr>
<td>Glass syringes - 5 ml</td>
<td>Tool</td>
<td>Fisher</td>
<td>14-823-10 B</td>
<td>&nbsp;</td>
<tr>
<td>Metal bridge - 18G</td>
<td>Tool</td>
<td>Fisher</td>
<td>14-825-17L</td>
<td>&nbsp;</td>
<tr>
<td>Luer Lok plastic syringes - 3 ml</td>
<td>Tool</td>
<td>Becton Dickinson</td>
<td>309585</td>
<td>&nbsp;</td>
<tr>
<td>Needles - 20G 1 1/2</td>
<td>Tool</td>
<td>Fisher</td>
<td>14-826-5C</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>
	

induction and clinical scoring of chronic-relapsing experimental autoimmune encephalomyelitis

<p class="jove_content">Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) that commonly affects young adults. It is characterized by demyelination and glial scaring in areas disseminated in the brain and spinal cord. These lesions alter nerve conduction and induce the disabling neurological deficits that vary with the location of the demyelinated plaques in the CNS (e.g. paraparesis, paralysis, blindness, incontinence).</p>
<p class="jove_content">Experimental autoimmune encephalomyelitis (EAE) is a model for MS. EAE was first induced accidentally in humans during vaccination against rabies, using viruses grown on rabbit spinal cords. Residues of spinal injected with the inactivated virus induced the CNS disease. Following these observations, a first model of EAE was described in non-human primates immunized with a CNS homogenate by Rivers and Schwenther in 1935. EAE has since been generated in a variety of species and can follow different courses depending on the species/strain and immunizing antigen used. For example, immunizing Lewis rats with myelin basic protein in emulsion with adjuvant induces an acute model of EAE, while the same antigen induces a chronic disease in guinea pigs.</p>
<p class="jove_content">The EAE model described here is induced by immunizing DA rats against DA rat spinal cord in emulsion in complete Freund's adjuvant. Rats develop an ascending flaccid paralysis within 7-14 days post-immunization. Clinical signs follow a relapsing-remitting course over several weeks. Pathology shows large immune infiltrates in the CNS and demyelination plaques. Special considerations for taking care for animals with EAE are described at the end of the video.</p>

Watch this Scientific Journal Video about Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis at JoVE.com

Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis

<p class="jove_content">This video demonstrates the induction and clinical scoring of an animal model of multiple sclerosis: chronic-relapsing experimental autoimmune encephalomyelitis in DA rats. The disease, induced by immunizing rats with an emulsion containing whole rat spinal cord and complete Freund's adjuvant, presents clinical signs resembling the human disease.</p>

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) that commonly affects young adults. It is characterized by ...

Research

JoVE Journal

Biology

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of ...

Assay for Neural Induction in the Chick Embryo

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

The Ladder Rung Walking Task: A Scoring System and its Practical Application.

Progress in the development of animal models for/stroke, spinal cord injury, and other neurodegenerative disease requires tests of high sensitivity to ...

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca<sup>2+</sup> Leak Channels in the ER Membrane and their Regulatory Mechanisms

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

In mammalian cells, the endoplasmic reticulum (ER) plays a key role in protein biogenesis as well as in calcium signalling1. The heterotrimeric Sec61 ...

Induction and Testing of Hypoxia in Cell Culture

Hypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells.  This decrease of oxygen tension can be due to a reduced supply in ...

Ovariectomy and 17&beta;-estradiol Replacement in Rats and Mice: A Visual Demonstration

Ovariectomy and 17&#946;-estradiol Replacement in Rats and Mice: A Visual Demonstration

Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are ...

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

The most common software analysis tools available for measuring fluorescence images are for two-dimensional (2D) data that rely on manual settings for ...

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

KCNE genes encode for a small family of Kv channel ancillary subunits that form heteromeric complexes with Kv channel alpha subunits to modify their ...

Induction and Analysis of Epithelial to Mesenchymal Transition

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Population and family-based genetic studies typically result in the identification of genetic variants that are statistically associated with a clinical ...