Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

1:37

Preparation of Cell Cytosol Containing Functional Glucocorticoid Receptor

3:04

Immunoabsorption of GR from the Cytosol and Dissociation of hsp90

5:16

Reconstitution of GR-hsp90 Heterocomplex using Molecular Chaperones, Cofactors, and ATP

6:17

Analysis of Reconstituted Protein Complexes and Ligand Binding Activity

8:26

Representative GR-hsp90 Heterocomplex Formation and Steroid Binding Activity

10:04

Conclusion