Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
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11:07 min
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September 21st, 2011
DOI :
September 21st, 2011
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Chapters in this video
0:05
Title
1:37
Preparation of Cell Cytosol Containing Functional Glucocorticoid Receptor
3:04
Immunoabsorption of GR from the Cytosol and Dissociation of hsp90
5:16
Reconstitution of GR-hsp90 Heterocomplex using Molecular Chaperones, Cofactors, and ATP
6:17
Analysis of Reconstituted Protein Complexes and Ligand Binding Activity
8:26
Representative GR-hsp90 Heterocomplex Formation and Steroid Binding Activity
10:04
Conclusion
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