<h2 class="jove_title"><a href="https://www.jove.com/video/3709">Hybridization in situ of salivary glands, ovaries and embryos of vector mosquitoes</a></h2>
Jennifer Juhn1, Anthony A. James2, 1 1Department of Molecular Biology and Biochemistry, University of California, Irvine , 2Department of Microbiology and Molecular Genetics, University of California, Irvine 
Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.
<h2 class="jove_title"><a href="https://www.jove.com/video/3763">Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos</a></h2>
Maureen Sinclair1, Jun-Yong Huang2, 1 1Institute for Cell and Molecular Biosciences, University of Newcastle, United Kingdom, 2Institute for Cell and Molecular Biosciences, University of Newcastle
The kinetochore is where the SAC initiates its signal monitoring the mitotic segregation of the sister chromatids. A method is described to visualize the recruitment and turnover of one of the kinetochore proteins and its coordination with the chromosome motion in Drosophila embryos using a Leica laser scanning confocal system.
<h2 class="jove_title"><a href="https://www.jove.com/video/3765">A simplified technique for In situ excision of cornea and evisceration of retinal tissue from human ocular globe</a></h2>
Mohit Parekh1, Stefano Ferrari1, Enzo Di Iorio1, Vanessa Barbaro1, Davide Camposampiero1, Marianthi Karali2, Diego Ponzin1, Gianni Salvalaio3, 1 1Fondazione Banca Degli Occhi del Veneto O.N.L.U.S. , 2Telethon Institute for Genetics &#38; Medicine (T.I.G.E.M.), 3Fondazione Banca Degli Occhi del Veneto, O.N.L.U.S.
The paper describes a simplified technique to excise corneal and to eviscerate retinal tissues from the ocular globe of human cadaveric donors. The technique described here will help to excise good quality tissues to be used for transplantation, surgical or research purposes without damaging other tissues of the ocular globe.
<h2 class="jove_title"><a href="https://www.jove.com/video/3813">Efficient differentiation of mouse embryonic stem cells into motor neurons</a></h2>
Chia-Yen Wu1, Dosh Whye2, 1, Robert W. Mason1, Wenlan Wang1 1Nemours Biomedical Research, Alfred I. duPont Hospital for Children, 2Department of Pediatrics, Columbia University Medical Center
We developed a new protocol to improve efficiency of in vitro differentiation of mouse embryonic stem cells into motor neurons. The differentiated ES cells acquired motor neurons features as evidenced by expression of neuronal and motor neuron markers using immunohistochemical techniques.
<h2 class="jove_title"><a href="https://www.jove.com/video/3847">Corneal Donor Tissue Preparation for Endothelial Keratoplasty</a></h2>
Maria A. Woodward1, Michael Titus2, Kyle Mavin2, Roni M. Shtein1 1Department of Ophthalmology, University of Michigan , 2MidWest Eye Banks
Endothelial corneal transplantation is a surgical technique for treatment of posterior corneal diseases. Mechanical microkeratome dissection to prepare tissue results in thinner, more symmetric grafts with less endothelial cell loss and improved outcomes. Dissections can be performed at the eye bank prior to corneal transplantation surgery.
<h2 class="jove_title"><a href="https://www.jove.com/video/3958">Endothelialized microfluidics for studying microvascular interactions in hematologic diseases</a></h2>
David R. Myers1, 2, 3, 4,*, Yumiko Sakurai1, 2, 3, 4,*, Reginald Tran1, 2, 3, 4, Byungwook Ahn1, 2, 3, 4, Elaissa Trybus Hardy1, 2, 3, 4, Robert Mannino1, 2, 3, 4, Ashley Kita1, 2, 3, 4, Michelle Tsai1, 2, 3, 4, Wilbur A. Lam1, 2, 3, 4 1Department of Pediatrics, Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children&#39;s Healthcare of Atlanta , 4Winship Cancer Institute of Emory University * These authors contributed equally
A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (&lt;30 &mu;m) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.
<h2 class="jove_title"><a href="https://www.jove.com/video/3993">Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping</a></h2>
N. Jeremy Hill1, Disha Gupta2, 1, Peter Brunner2, 1, Aysegul Gunduz2, 1, Matthew A. Adamo3, Anthony Ritaccio2, Gerwin Schalk1, 2, 4, 5, 6, 7 1Wadsworth Center, New York State Department of Health, 2Department of Neurology, Albany Medical College, 3Department of Neurosurgery, Albany Medical College, 4Department of Neurosurgery, Washington University, 5Department of Biomed. Eng., Rensselaer Polytechnic Institute, 6Department of Biomed. Sci., STATE UNIVERSITY OF NEW YORK AT ALBANY, 7Department of Elec. and Comp. Eng., University of Texas at El Paso 
We present a method for collecting electrocorticographic signals for research purposes from humans who are undergoing invasive epilepsy monitoring. We show how to use the BCI2000 software platform for data collection, signal processing and stimulus presentation. Specifically, we demonstrate SIGFRIED, a BCI2000-based tool for real-time functional brain mapping.

june 2012: this month in jove

早在1905年，在现在的捷克共和国，爱德华Zirm进行角膜移植手术，角膜移植，角膜损伤失明病人恢复视力。今天，世界各地的眼库准备，存储，分发，所以可以每年，成千上万的视线节能keratoplasties医院捐献眼角膜。 2012年6月，朱庇特的有两个研究组，一组来自意大利和其他来自密歇根州的，谁表现出两个不同的方法角膜移植准备移植前它的眼睛。 我们从意大利的作者向我们展示他们的技术，为从全球眼，其中涉及一系列消毒准备无菌处理的解决方案，在第一次扣篮，创建一个切口巩膜表面上，并最终分离角膜巩膜轮辋切除角膜远离地球。一旦被删除，角膜内皮细胞密度和活力，是checked和它准备长期储存，在此期间，媒体定期在高通量的方式与其他移植测试敷在眼库。 在全层角膜移植手术，移植被称为穿透性角膜移植过程中使用，然而，朱庇特了解到，在一些患病的眼角膜内皮细胞层影响。我们从中西部眼库的作者展示了捐献角膜后弹力剥离自动化内皮角膜移植术（DSAEK），移植角膜内皮细胞层的过程，涉及进一步的处理。这比较近的过程可以通过使用角膜 - 通过角膜内皮细胞层，使可用于移植的分离装置，可以精确切割。使用裂隙灯和反射镜，捐助组织的统一性和质量验证和移植角膜移植苏存储rgery。 在一起，两组作者从不同的大洲，记录消除眼睛的整个角膜和孤立的内皮角膜移植后层的步骤 - 一个是降低感染或排斥反应的风险和提高看病人的能力的过程。 在朱庇特的神经，多学科的临床医生和科学家团队，引进新的脑电图，或脑电图研究中的应用。脑电图是一种侵入性的过程，使用直接放置在大脑中的电极带和网格定位在癫痫患者发作灶。这个过程也可以用来映射需要进行切除手术过程中幸免的皮层区域。皮层的映射是通过监测是否在运动或演讲的中断给定的电极结果刺激。患者通常受到颅内监测定位扣押灶ABOUT一个星期，这时间的机会，为研究人员研究人类大脑的脑电图，具有更好的信号噪声性能和较少的易感性比非侵入性脑电图或脑电记录文物的行动提供了一个独特的窗口。 经过简单的认知，或机动任务期间，确定基线活动休息时，我们的作家记录在高伽玛频率范围的脑活动。然后，这些调查表明如何使用SIGFRIED的软件系统进行快速，实时的基于脑电图信号，这是进一步分析提供的信息与特定任务相关的大脑区域的功能定位。 朱庇特在生物工程中，遇到一队科学家表明，微血管大约可以在一个芯片上重新。这种“芯片”，实际上是一个与内皮细胞表面的微通道设备。苏8 photolithog的raphy用于硅片，作为一个PDMS模具上蚀刻渠道模式。一旦设备已制作，它是播种与血管内皮细胞，在培养的渠道。 PDMS的透明度，细胞可以通过显微镜成像。这在体外微血管模型提供一个受控的微环境，可用于正常的血流动力学过程，以及在血液病研究。这个简短的总结是在一些Jove的六月份的内容仅仅一瞥。进一步的调查可能会导致在成蚊组织和胚胎原位杂交方法，观察果蝇的有丝分裂，并分化成运动神经元细胞胚胎茎。敬请关注。

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June 2012: This Month in JoVE

2012年6月：本月在朱庇特

Back in 1905, in what is now the Czech Republic, Eduard Zirm performed the first corneal transplantation surgery (keratoplasty), which restored vision to ...