May 24th, 2013
•Intracellular Ca2+ dynamics are very important in sperm physiology and Ca2+-sensitive fluorescent dyes constitute a versatile tool to study them. Population experiments (fluorometry and stopped flow fluorometry) and single cell experiments (flow cytometry and single cell imaging) are used to track spatio-temporal [Ca2+] changes in human sperm cells.
Tags
Related Videos
Techniques for Imaging Ca2+ Signaling in Human Sperm
Flow Cytometry Purification of Mouse Meiotic Cells
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
LED Thermo Flow — Combining Optogenetics with Flow Cytometry
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts