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Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

20.2K Views

19:26 min

May 24th, 2013

DOI :

10.3791/50344-v

May 24th, 2013


Transcript

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Ca2 Signaling

Chapters in this video

0:05

Title

2:38

Sperm Sample Preparation by the Swim-up Method

4:52

Fluorescent Dye Loading for Ca2+ Measurements

5:41

Conventional Fluorometry

6:59

Stopped Flow Fluorometry

8:30

Flow Cytometry

9:40

Single Cell Imaging

12:11

Results: Progesterone Induces Intracellular Ca2+ Changes in Human Sperm Detected by Fluorometric Techniques

17:59

Conclusion

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