<a href="https://www.jove.com/video/4003">诱导多能干细胞来源于小鼠的生成</a> 迈克尔·J·博兰1，珍妮弗L·哈森，家克里斯托L. Nazor 1，阿尔贝托R.罗德里格斯2，格雷格·马丁2，谢尔盖·Kupriyanov 2，克里斯汀K.鲍德温1 1多里斯神经科学中心细胞生物学系，斯克里普斯研究所，2小鼠基因核心设施，斯克里普斯研究所生成诱导多能干细胞（IPSC）线生产线，不同的发展潜力，即使当他们通过标准测试的多能性。在这里，我们描述了一个协议产生小鼠iPSCs的，它定义了IPSC的线，拥有完整的多能性完全来自。 <a href="https://www.jove.com/video/50016">分析小鼠</a>雪旺氏细胞轴突生长沿江开发 斯蒂芬Heermann 1，2，1，3克斯廷Krieglstein 分子胚胎学，解剖学研究所和细胞生物学，弗莱堡大学，海德堡大学神经解剖学杂志，3弗里亚斯，弗赖堡大学在这里，我们描述了雪旺氏细胞（SC）迁移试验中，SC的方向发展，延长轴突。 <a href="https://www.jove.com/video/50027">一个轻量级的，耳机为基础的系统操作鸣禽听觉反馈</a> :卢卡斯A.霍夫曼1，2，康纳尔W.凯利1，3，大卫·尼克尔森1，2，塞缪尔J.清醒的1 1，埃默里大学生物系，神经科学研究生课程2，埃默里大学，3编RAM中，埃默里大学神经科学和行为生物学我们描述了:适合更换一个鸣禽的自然听觉反馈与一个操作的声学信号的微型耳机的设计和组装。在线无害化处理硬件是用来控制歌曲的输出，通过耳机听觉反馈引入实时的错误，电机驱动声乐学习。 <a href="https://www.jove.com/video/50086">对黑色素瘤修饰符一个斑马鱼原地肿瘤模型的筛选</a> 1，2，3，Houvras Yariv先生克雷格·J.中企视窗1 Sharanya艾扬格 1程序，马萨诸塞州医学院，大学分子医学和癌症生物学系，威尔康乃尔医学院的手术费和医药部门，3个部门的手术费和医药，纽约长老会医院 <a href="https://www.jove.com/video/50117">使用滑翔分析生物大分子的抗弯刚度测量</a> 道格拉斯S.马丁，陆羽，布赖恩L.·Hoozen 劳伦斯大学物理系， 持效期长或生物大分子的抗弯刚度的方法来衡量。该方法使用一个动蛋白驱动的微管的滑动测定试验方法来测定的持久性个别微管长度和是适应actin为基础的滑翔检测。&lt;<html> / P&gt; <a href="https://www.jove.com/video/50123">从环境中自然产生的趋磁细菌的采集，分离和富集</a> 1，史蒂芬K.低1，2，威麟3，布赖恩·H.较低的2 Zachery Oestreicher 1，美国俄亥俄州立大学地球科学，环境和自然资源，俄亥俄州立大学，地质与地球物理研究所，中国科学院科学学院的学校我们展示了一个方法，可以应用到天然水域中收集的趋磁细菌（MTB）。 MTB可以从沉积物样品，用一个相对简单的设置，利用细菌的天然磁分离和富集。隔离MTB然后可以同时使用光学和电子显微镜详细检查。 8"&gt; Transnuclear的小鼠与预先定义的T细胞受体的特异性抗弓形虫获得通过体细胞核移植 奥克塔伊Kirak，伊娃玛丽亚Frickel的Hidde的L. Ploegh 1，3 1，的Gijsbert M. Grotenbreg 1，2，鲁道夫詹尼士1，1，3，徐Heikyung 1，怀特黑德生物医学研究所，2部微生物学与生物科学学院，新加坡国立大学，麻省理工学院生物系我们在这里展示，通过体细胞核移植（SCNT）的表观遗传修饰可以作为一个工具来生成预先定义的T细胞受体（TCR）特异性的小鼠模型。这些transnuclear小鼠表达​​内源性基因座的内源启动子的控制下，从它们对应的TCR。

没有利益冲突的声明。

november 2012: this month in jove

在这个问题上，Oestreicher 等。我们展示了如何分离趋磁细菌从淡水样品，并集中在细菌的玻璃毛细管的一端。趋磁细菌然后可以被可视化的光和透射电子显微镜，用于其他各种测定。 此外，在2012年11月发行，博兰等。演示了如何以成纤维细胞重新编程为诱导多能干细胞（iPSCs），以及如何隔离iPSC的注入四倍体囊胚，如以前在<a href="http://www.jove.com/video/2168/transnuclear-mice-with-pre-defined-t-cell-receptor-specificities">朱庇特</a> 。口移液一般不推荐使用，因为它可以产生不利影响，该协议要求的技术，仔细执行，操作的胚胎。这是一个罕见的情况下，它实际上是确定在实验室中口移液管。如果诱导细胞重新全面多能，他们可能会导致完全由iPS细胞的小鼠在足月。 在JOVE神经科学中，Heermann和Krieglstein展示可视化雪旺氏细胞的发育沿轴突生长的方法。要做到这一点，我们的作家文化颈椎神经植上胶原基质，和治疗神经生长因子或其他物质的外植体。的胶原蛋白凝胶，然后可以被可视化的荧光或明视野显微镜，使用延时成像迁移部向周边部沿着轴突。 此外，在朱庇特神经，霍夫曼等人，把微小的耳机上的鸣禽研究他们如何使用听觉反馈来调整他们的歌声。作者展示了如何构建耳机，并将它们附加到鸟的头部，然后，他们可以通过调整声信号，研究鸟类的发声学习中的计算和神经生理学的基础上。 在JOVE临床中转化医学，艾扬格等。使用斑马鱼瘤模型研究基因，可以修改黑色素瘤的发病机制。这是通过首先创建的利益表达基因的转基因斑马鱼。然后进行各种检测，以研究不同的基因如何影响黑色素瘤的发病，侵袭和可移植性。 以JOVE生物工程，Martin 等人展示出一种滑行试验，以测量生物聚合物（例如微管）的抗弯刚度。 ，我们的作者可以通过将马达蛋白在显微镜幻灯片，并加入荧光标记的微管骨架聚合物的动态分析。 这个预览总结了几个显着的视频，文章在2012年11月朱庇特的问题。在整个11月的停止这些文章和许多检查出的全长版本。

Watch this Scientific Journal Video about November 2012: This Month in JoVE at JoVE.com

November 2012: This Month in JoVE

在这个问题上，Oestreicher<em&gt;等。</em&gt;我们展示了如何从淡水样品中分离出趋磁细菌的细菌集中在一端的玻璃毛细管。趋磁细菌然后可以被可视化的光和透射电子显微镜，用于其他各种测定。

2012年11月:这个月在朱庇特

In this issue, Oestreicher et al. show us how to isolate magnetotactic bacteria from freshwater samples, and concentrate the bacteria at one end of a ...

Research

JoVE Journal

Biology

9.5K 次观看.  Massachusetts Eye and Ear. 在这个问题上，Oestreicher等。我们展示了如何从淡水样品中分离出趋磁细菌的细菌集中在一端的玻璃毛细管。趋磁细菌然后可以被可视化的光和透射电子显微镜，用于其他各种测定。

视频: November 2012: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

2012年8月:本月在朱庇特

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

科研

生物学

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012年:年度回顾

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

2012年11月:这个月在朱庇特

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2012年8月:本月在朱庇特

2012年:年度回顾