This work was supported by The National Institute of Health (NIH-2RO1NS033661) and by the Alabama Agricultural Experiment Station (HATCH ALA021-1-09017).

注：此处进行的所有手术均由IACUC（研究合规办公室）提交给批准，并进行以下NIH的指导方针。在行为测试模式用于小鼠幼稚，不用于其他测试。的C57BL / 6野生型和敲除小鼠在此协议中使用先前已被描述图9和这里介绍的数据是从该手稿。 1.准备的检测室和开放现场设备<ol><li>使用多单元旷场迷宫（OFM）包括四个活动室中被用于此分析（ 图1）。每个腔室测定50厘米（长）×50厘米（宽）×38厘米（高度），并从白色高密度和非多孔塑料被做。 </li><li>走动期间质感迷宫为牵引的地板，而迷宫墙壁光滑。迷宫象限是完全空了本次测试的目的。在审议这一原的其余部分栏，上述的迷宫的单个象限会被利用来演示OFM。 </li><li>擦拭室在使用前95％的乙醇和之前随后的测试，以除去由先前对象小鼠左任何气味线索。 </li><li>允许乙醇完全蒸发之前，测试小鼠。这可能需要每个测试环节之间的5-10分钟。 </li><li>为了便于分析，使用SMART视频跟踪软件PanLab /哈佛仪器来记录和评估鼠标移动。 注意：任何商用视频跟踪摄像机和软件可以被用来跟踪测试受试者和评估从开启字段迷宫结果。这是非常重要的是，最终用户要了解如何校准和运行用于每个单独的分析软件。不管摄像机和用来跟踪软件的，当相机和软件根据制造商的说明被正确校准得到最好的结果。 </li><li>执行测试我适用标准昏暗的房间能容纳迷宫设备和运行软件所需的计算机。或者通过将其连接到在天花板上，或使用任何升高的支持系统，它允许照相机的镜头看到整个迷宫区域（ 图2）暂停所述视频摄像机的迷宫上方。 </li><li>由于人类管理员的考验，一定要在房间足够的空间是在迷宫测试科目完全不可见，以免影响小鼠的行为。 </li></ol> 2.准备软件测量活动<ol><li>打开视频跟踪软件。 </li><li>一旦软件被打开，将光标移动到位于“数据采集”选项卡，只需单击打开此选项下的“单对象跟踪”选项。 </li><li>选择位于屏幕底部的“静态背景”选项。 </li><li>选择后，“静态背景”的选择N，有必要使用软件采取迷宫的图像之前加入的测试对象。要做到这一点，将光标移动到“照片”按钮位于屏幕，只需点击一下下方。 注：该软件将利用场景的一个图象，而不测试受试者将被从在跟踪处理拍摄的图像中减去。这导致仅被检体的运动由软件进行分析。 </li><li>确认上述拍摄的背景图像完全被跟踪软件通过移动光标到“测试”按钮位于屏幕的底部，并点击一次取出。固体白场将被显示，如果背景图像从跟踪图像完全除去。如果照明条件发生变化或迷宫被意外移动，你会看到在这个领域黑“阴影”，表示两个图像不完全一致。为了改变这种情况，只需重复STE上面的P 2.4。 </li><li>确认的背景设置后，使用时间选项来配置采集过程中时间控制的方式。要做到这一点，将光标移动到“配置”选项卡并单击一次“计时”的标题。使用新打开的窗口进入实验参数。 </li><li>对于这个协议，选择了“设定时间”选项，因为10分钟的跟踪期。设置了“延迟期”，5秒，以允许用户的时间来将鼠标在迷宫的中心，并事先以跟踪的起始搬走。进入10分钟的“采集时间”为测试的持续时间。将“停止控制”设置为“当设定的时间（10分钟）结束”，这将自动关闭摄像头和软件的跟踪功能。 </li><li>将光标移动到“关闭”按钮后，所有的计时已设置为关闭该窗口。你现在就可以开始测试荷兰国际集团的过程。 </li></ol> 3.管理的开放式现场测试注：在本协议中所使用的软件允许多达16个独立的小鼠的跟踪一次。为便于完成并且如上所述，这里所讨论的协议是使用OFM的单个象限一个鼠标。在使用这种协议的设备，最多4个独立的小鼠可以用迷宫的每个象限进行跟踪。如果利用多机箱迷宫，将第一对象小鼠在其定义的象限后，将剩余的小鼠成各自的迷宫象限用于跟踪分析。对于该协议的目的，进一步说明将具体到迷宫的单象限。 <ol><li>从他们的住房房间带入考场带来老鼠在他们的家笼。允许小鼠之前适应的程序空间最少30分钟以开始测试。 </li><li>删除单个米轻轻抓住它的尾巴从家笼乌斯和鼠标放置在野外露天迷宫的中间，而同时启动由单一的点击SMART软件上的开始按钮，开始跟踪鼠标移动。这是正常的鼠标快速移动到迷宫的外围墙壁和释放的时间和跟踪鼠标捕获应一致，以记录这个动作。 </li><li>允许在整个迷宫的各象限中的对象小鼠的自由和不间断运行为一个10分钟的时间，在此期间，跟踪软件将记录的运动（ 图3）。 </li><li>在测试期结束时，拿起对象小鼠轻轻，从迷宫中取出，并将其返回到其家笼中。 </li><li>前清洗迷宫，目视计数存在于迷宫粪便博利粒料和手动记录号码进行进一步的分析。 </li><li>删除所有粪粒，并擦干净排尿所有景点。喷地板与迷宫象限用95％乙醇的壁并用干净的纸巾擦拭。让乙醇溶液测试其他老鼠之前完全干燥。 </li><li>下一个鼠标重复此过程。 </li></ol> 4.测量与行为分析在测试过程注：对于测量，露地行为三个方面使用该协议（见讨论）容易表征。关于如何访问视频追踪软件这些测量结果的简要说明如下。 <ol><li>从SMART软件的主界面，将光标移动到“区”选项卡，只需单击“定义”，打开区编辑器。 </li><li>按照SMART软件用户手册中的详细说明，也定义区域或网格叠加的跟踪路径。这里，该软件被用来定义一个5×5网格10厘米的正方形覆盖迷宫的地板（ <strong&gt;的图4）。一定要创建的区域文件保存关闭区编辑之前。 </li><li>从SMART软件的主界面，将光标移动到“分析”选项卡和单单击打开数据分析窗口。 </li><li>将光标移动到“文件”选项卡，然后打开上面创建的区域文件。 </li><li>将光标移动到“配置”选项卡，打开“轨迹分析”选项。这将打开“单科分析配置”窗口。 <ol><li>将光标移动到“标准”选项卡，并从可用参数框（左侧）将“旅行描述休闲，距离”参数设置为包含参数框（右侧）。 </li><li>将光标移动到“过渡区”选项卡，将所有适当的参数包括该参数框上面。 </li><li>确保“全田径”框在窗口底部检查。 </li><li>莫已经光标到OK按钮并关闭单科分析配置窗口。 注：根据您要进行分析，许多其他的选择可以在该窗口中选择从分析挖掘数据。看了你的具体方案的详细用户手册，以确定哪些参数是为您的数据分析最重要的。 </li></ol></li><li>在程序窗口的左上角的文件选项卡，打开单科轨道窗口，放置一个复选标记旁边正在分析所有曲目。将光标移动到复选标记按钮，​​在窗口的顶部，然后单击关闭轨道资源管理器窗口。 </li><li>将光标移动到数据分析窗口中的“开始”按钮，然后点击一个启动轨道数据进行分析。 </li><li>分析数据可以作为任一ASCII文本文件输出，或者它可以直接导出到Excel电子表格。使用软件程序正在使用的输出工具来输出达TA供自己使用。 注：总行驶距离和时间，指示区花费将输出以下以上概述的数据的分析步骤。再次，应当强调的是该步骤以达到这些测量此处表示将根据所使用的用户软件不同。但数据本身和结果的解释应该是相似的使用的软件程序无关。还值得一提的是，测试管理员偏压从该协议作为所有收集的定量由软件而不是管理员测量数据中的数据中删除。因此没有qualifiable元件到所述收集的数据。 </li></ol>

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The authors have nothing to disclose.

旷场迷宫是在动物行为学研究中最广泛使用的平台之一。一些重要的常规和行为学参数2,4可以收集和OFM的表现时进行分析。这些数据允许研究者测量行为，从整体运动活性焦虑有关的情感行为8。然而，使用OFM的也不是没有缺点。一个混杂的问题是大范围可以在任何测试期间被操纵的静态变量。例子包括时间，照明条件和新的物体包容。变异性在实验方案的设置和设计，这是必要?...

The Open Field Maze (OFM) was initially developed in 1934 as a test to measure emotionality in rodents1. It has attained the status of being one of the most widely used measures of behavior in animal psychology2. It provides an easy and fairly rapid assessment of well-defined behaviors requiring no training to the test subject and little to no specialized training for the human administering the test. These attributes have led to wide-spread use of the open field maze in research extended to other animal species such as calves, pigs, rabbits, primates, honeybees and lobsters3. Part of its popularity arises from the fact that the psychological and physiological concepts underlying the tests are generally straight-forward and well understood. For example, it has been postulated that evolutionary forces have selected for a common response in animals such that most species display anxiety-mediated fear or flight responses to specific stimuli. Rodents for example, show distinct aversions to large, brightly lit, open and unknown environments4. We can assume they have been phylogenetically conditioned to see these types of environments as dangerous. All of these features are incorporated in the open field maze and form the basis of its use in behavioral paradigm testing.
An open field maze consists of a wall-enclosed area that is of sufficient height to prevent the subject from escaping. Typical maze shapes are circular or square with an area large enough, based on the size of the subject tested, to elicit a feeling of openness in the center of the maze. A number of variables can be scored in the open field maze with most parameters involving differing types of motor activity2. Ambulation is the most common behavior studied but others such as latency or rearing can also be measured. Most often, rodent behavior is analyzed in a bare maze. However, the addition of objects, either one or many to the maze floor, adds the ability to see how the subject interacts with novel additional stimuli2. Relevant parameters when objects are presented are typically the number of approaches to an object or in some cases, preference or aversion for one object over another.
Many behavioral tests of anxiety are based on the subject animal&#8217;s body activity and locomotion5. Interpreting behavioral tests for emotionality while separating non-emotional confounding factors, such as motor activity, has been the subject of intense debate6,7. As the OFM was originally described, two measures of emotionality can be deduced, locomotor activity and fecal boli deposits or defecation1. However, these two measures have been shown in some studies to be unrelated supporting the conclusion that emotionality in rodents is multidimensional5. Regardless, discrepancies in the literature regarding these measures and emotionality or anxiety in mouse models may be attributed to differences in analysis criteria or differences in testing procedures. Studies have conclusively linked results from OFM analysis with other measures of anxiety when comparing mouse models8.

<table border="0" cellpadding="0" cellspacing="0" width="631">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:160px;">Multi Unit Open Field Test</td>
			<td style="width:171px;">San Diego Instruments, Inc.</td>
			<td style="width:109px;">White 7001-0354</td>
			<td style="width:192px;">Any single or multi unit open field maze can be used</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:160px;">SMART DT Tracking Software</td>
			<td style="width:171px;">PanLab/Harvard Apparatus</td>
			<td style="width:109px;">76-0695</td>
			<td style="width:192px;">Any tracking software can be utilized with this protocol</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:160px;">Sony 990x Video Camera Recorder</td>
			<td style="width:171px;">Sony</td>
			<td style="width:109px;">CCD-TRV328</td>
			<td style="width:192px;">Any suitable video camera can be attached to computer for recording tracking profiles.</td>
		</tr>
	</tbody>
</table>

use of the open field maze to measure locomotor and anxiety-like behavior in mice

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

每在大多数情况下，测试小鼠的品系的个体的平均数目是约20，以产生足够的统计相关性。然而，该数量可以在8-30的范围内根据对小鼠的可用性。根据所需要的测量或比较，它也有利于使用年龄匹配的主题。 第一，可以说是最重要的具体的参数在田野迷宫来衡量的总门诊距离。同时测量单位无关为比较目的，它是最经常表达为公制测量（厘米）。在这里（ 图5）?...

Watch this Scientific Journal Video about Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice at JoVE.com

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

使用开放式现场迷宫来衡量小鼠的运动能力和焦虑样行为

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of ...

use-open-field-maze-to-measure-locomotor-anxiety-like-behavior

Research

JoVE Journal

Behavior

71.8K 次观看.  Auburn University. A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

视频: Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Shallow Water (Paddling) Variants of Water Maze Tests in Mice

When Richard Morris devised his water maze in 19817, most behavioral work was done in rats. However, the greater understanding of mouse genetics led to the mouse becoming increasingly important. But researchers found that some strains of mutant mice were prone to problems like passively floating or diving when they were tested in the Morris water maze11. This was unsurprising considering their natural habitat; rats swim naturally (classically, the "sewer rat"), whereas mice evolved in the dry areas of central Asia.
To overcome these problems, it was considered whether shallow water would be a sufficient stimulus to provide escape motivation for mice. This would also avoid the problems of drying the small creatures with a towel and then putting them in a heated recovery chamber to avoid hypothermia, which is a much more serious problem than with rats; the large ratio of surface area to volume of a mouse makes it particularly vulnerable to rapid heat loss.
Another consideration was whether a more natural escape strategy could be used, to facilitate learning. Since animals that fall into water and swim away from the safety of the shore are unlikely to pass on their genes, animals have evolved a natural tendency to swim to the edge of a body of water. The Morris water maze, however, requires them to swim to a hidden platform towards the center of the maze - exactly opposite to their evolved behavior. Therefore the paddling maze should incorporate escape to the edge of the apparatus. This feature, coupled with the use of relatively non-aversive shallow water, embodies the "Refinement" aspect of the "3 Rs" of Russell and Burch8.
Various types of maze design were tried; the common feature was that the water was always shallow (2 cm deep) and escape was via a tube piercing the transparent wall of the apparatus. Other tubes ("false exits") were also placed around the walls but these were blocked off. From the inside of the maze all false exits and the single true exit looked the same. Currently a dodecagonal (12-sided) maze is in use in Oxford, with 12 true/false exits set in the corners. In a recent development a transparent paddling Y-maze has been tested successfully.

Shallow Water Paddling Variants of Water Maze Tests in Mice

Mice can swim, but many strains appear to find this activity stressful. To overcome this problem mazes have been devised where escape from shallow water is used to motivate behaviour. These have been demonstrated to support learning at least as good as the traditional and widely used Morris water maze.

浅水（杖刑）的变体在小鼠体内的水迷宫测试

When Richard Morris devised his water maze in 19817, most behavioral work was done in rats. However, the greater understanding of mouse genetics led to ...

科研

行为学

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

We present an operant system for the detection of pain in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) assesses pain behaviors in a more clinically relevant way by not relying on reflex-based measures of nociception. Food fasted, hairless (or shaved) rodents are placed into a Plexiglas chamber which has two Peltier-based thermodes that can be programmed to any temperature between 7 &deg;C and 60 &deg;C. The rodent is trained to make contact with these in order to access a reward bottle. During a session, a number of behavioral pain outcomes are automatically recorded and saved. These measures include the number of reward bottle activations (licks) and facial contact stimuli (face contacts), but custom measures like the lick/face ratio (total number of licks per session/total number of contacts) can also be created. The stimulus temperature can be set to a single temperature or multiple temperatures within a session. The OPAD is a high-throughput, easy to use operant assay which will lead to better translation of pain research in the future as it includes cortical input instead of relying on spinal reflex-based nociceptive assays.

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

We present a user-friendly, high-throughput operant system for the evaluation of pain behaviors in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) can assess pain through a reward/conflict paradigm thus providing a more humane way of testing. This protocol will yield more clinically relevant and translational data from rodents.

使用操作性口面疼痛评估设备（OPAD）测量伤害行为的变化

We present an operant  system for the detection of pain in awake, conscious rodents. The Orofacial  Pain Assessment Device (OPAD) assesses pain behaviors ...

Use of an Eight-arm Radial Water Maze to Assess Working and Reference Memory Following Neonatal Brain Injury

Working and reference memory are commonly assessed using the land based radial arm maze.&#160;However, this paradigm requires pretraining, food deprivation, and may introduce scent cue confounds. The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs. Specifically, subjects are required to avoid the arms previously used for escape during each testing day (working memory) as well as avoid the fixed arms, which never contain escape platforms (reference memory). Re-entries into arms that have already been used for escape during a testing session (and thus the escape platform has been removed) and re-entries into reference memory arms are indicative of working memory deficits. Alternatively, first entries into reference memory arms are indicative of reference memory deficits. We used this maze to compare performance of rats with neonatal brain injury and sham controls following induction of hypoxia-ischemia and show significant deficits in both working and reference memory after eleven days of testing. This protocol could be easily modified to examine many other models of learning impairment.

The eight-arm radial water maze is designed to evaluate reference and working memory performance simultaneously by requiring subjects to use extra-maze cues to locate escape platforms and remedies the limitations observed in land based radial arm maze designs.

Working and reference memory are commonly assessed using the land based radial arm maze.&#160;However, this paradigm requires pretraining, food ...

Behavioral and Locomotor Measurements Using an Open Field Activity Monitoring System for Skeletal Muscle Diseases

The open field activity monitoring system comprehensively assesses locomotor and behavioral activity levels of mice. It is a useful tool for assessing locomotive impairment in animal models of neuromuscular disease and efficacy of therapeutic drugs that may improve locomotion and/or muscle function. The open field activity measurement provides a different measure than muscle strength, which is commonly assessed by grip strength measurements. It can also show how drugs may affect other body systems as well when used with additional outcome measures. In addition, measures such as total distance traveled mirror the 6 min walk test, a clinical trial outcome measure. However, open field activity monitoring is also associated with significant challenges: Open field activity measurements vary according to animal strain, age, sex, and circadian rhythm. In addition, room temperature, humidity, lighting, noise, and even odor can affect assessment outcomes. Overall, this manuscript provides a well-tested and standardized open field activity SOP for preclinical trials in animal models of neuromuscular diseases. We provide a discussion of important considerations, typical results, data analysis, and detail the strengths and weaknesses of open field testing. In addition, we provide recommendations for optimal study design when using open field activity in a preclinical trial.

Open field activity levels are used to assess locomotive and behavioral activity levels. This protocol provides a well-designed, standardized protocol to use in preclinical trials for neuromuscular disorders.

行为和运动测量使用一个开放的领域活动监控系统的骨骼肌疾病

The open field activity monitoring system comprehensively assesses locomotor and behavioral activity levels of mice. It is a useful tool for assessing ...

The Attentional Set Shifting Task: A Measure of Cognitive Flexibility in Mice

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many neuropsychiatric and neurodevelopmental disorders, including depression, post-traumatic stress disorder, schizophrenia and autism spectrum disorder. Deficits in cognitive function also represent the most difficult symptom domain&#160;to successfully treat, as serotonin reuptake inhibitors and tricyclic antidepressants have only modest effects. Functional neuroimaging studies and postmortem analysis of human brain tissue implicate the PFC as being a primary region of dysregulation in patients with these disorders. However, preclinical behavioral assays used to assess these deficits in mouse models which can be readily manipulated genetically and could provide the basis for studies of new treatment avenues have been underutilized. Here we describe the adaptation of a behavioral assay, the attentional set shifting task (AST), to be performed in mice to assess prefrontal cortex mediated cognitive deficits. The neural circuits underlying behavior during the AST are highly conserved across humans, nonhuman primates and rodents, providing excellent face, construct and predictive validity.

The goal of this protocol is to perform a behavioral assay such as the attentional set shifting task (AST) to assess prefrontal cortex-mediated cognitive flexibility in mice.

在注意定势转移任务：测量小鼠认知的灵活性

Cognitive impairment, particularly involving dysfunction of circuitry within the prefrontal cortex (PFC), represents a core feature of many ...

Place and Response Learning in the Open-field Tower Maze

This protocol describes how the Open-field Tower Maze (OFTM) paradigm is used to study spatial learning in rodents. This maze is especially useful for examining how rats learn to use a place- or response-learning to successfully navigate in an open-field arena. Additionally, this protocol describes how the OFTM differs from other behavioral maze paradigms that are commonly used to study spatial learning in rodents. The OFTM described in this article was adapted from the one previously described by Cole, Clipperton, and Walt (2007). Specifically, the OFTM was created to test spatial learning in rodents without the experimenter having to consider how &#8220;stress&#8221; might play a role as a confounding variable. Experiments have shown that stress-alone can significantly affect cognitive function1. The representative results section contains data from an experiment that used the OFTM to examine the effects of estradiol treatment on place- and response-learning in adult female Sprague Dawley rats2. Future studies will be designed to examine the role of the hippocampus and striatum in place- and response-learning in the OFTM.

The Open-field Tower Maze (OFTM) is a maze created to study the behavioral and neural mechanisms of spatial learning (e.g., place- or response-learning) in rats. This maze is especially useful for experimenters who want to use a non-stressful maze paradigm to investigate spatial learning in their research.

在打开场塔迷宫的地方，反应学习

This protocol describes how the Open-field Tower Maze (OFTM) paradigm is used to study spatial learning in rodents. This maze is especially useful for ...

Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria

Captive-rearing observations are valuable for revealing aspects of fish behavior and ecology when continuous field investigations are impossible. Here, a series of basic techniques are described to enable observations of the reproductive behavior of a wild-caught gobiid fish, as a model, kept in an aquarium. The method focuses on three steps: collection, transport, and observations of reproductive ecology of a substrate spawner. Essential aspects of live fish collection and transport are (1) preventing injury to the fish, and (2) careful acclimation to the aquarium. Preventing harm through injuries such as scratches or a sudden change of water pressure is imperative when collecting live fish, as any physical damage is likely to negatively affect the survival and later behavior of the fish. Careful acclimation to aquaria decreases the incidence death and mitigates the shock of transport. Observations during captive rearing include (1) the identification of individual fish and (2) monitoring spawned eggs without negative effects to the fish or eggs, thereby enabling detailed investigation of the study species' reproductive ecology. The subcutaneous injection of a visible implant elastomer (VIE) tag is a precise method for the subsequent identification of individual fish, and it can be used with a wide size range of fish, with minimal influence on their survival and behavior. If the study species is a substrate spawner that deposits adhesive eggs, an artificial nest site constructed from polyvinyl chloride (PVC) pipe with the addition of a removable waterproof sheet will facilitate counting and monitoring the eggs, lessening the investigator's influence on the nest-holding and egg-guarding behavior of the fish. Although this basic method entails techniques that are seldom mentioned in detail in research articles, they are fundamental for undertaking experiments that require the captive rearing of a wild fish.

A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.

鱼类生殖生态学研究的基本方法

Captive-rearing observations are valuable for revealing aspects of fish behavior and ecology when continuous field investigations are impossible. Here, a ...

Central and Divided Visual Field Presentation of Emotional Images to Measure Hemispheric Differences in Motivated Attention

Two dominant theories on lateralized processing of emotional information exist in the literature. One theory posits that unpleasant emotions are processed by right frontal regions, while pleasant emotions are processed by left frontal regions. The other theory posits that the right hemisphere is more specialized for the processing of emotional information overall, particularly in posterior regions.
Assessing the different roles of the cerebral hemispheres in processing emotional information can be difficult without the use of neuroimaging methodologies, which are not accessible or affordable to all scientists. Divided visual field presentation of stimuli can allow for the investigation of lateralized processing of information without the use of neuroimaging technology. 
This study compared central versus divided visual field presentations of emotional images to assess differences in motivated attention between the two hemispheres. The late positive potential (LPP) was recorded using electroencephalography (EEG) and event-related potentials (ERPs) methodologies to assess motivated attention. Future work will pair this paradigm with a more active behavioral task to explore the behavioral impacts on the attentional differences found.

This study compared central versus divided visual field presentations of emotional images to assess differences in motivated attention between the two hemispheres. The late positive potential (LPP) was recorded using electroencephalography (EEG) and event-related potentials (ERPs) methodologies to assess motivated attention.

中央和分割的视觉场表现的情绪图像, 以测量半球的差异, 激发注意

Two dominant theories on lateralized processing of emotional information exist in the literature. One theory posits that unpleasant emotions are processed ...

Testing Animal Anxiety in Rats: Effects of Open Arm Ledges and Closed Arm Wall Transparency in Elevated Plus Maze Test

The elevated plus maze test is a behavioral test for assessing animal anxiety in rodents. Although this test is widely applied in the field of behavioral science, conflicting outcomes are often provided from different laboratories. To identify reasons for the different outcomes, we previously focused on arm features, which differ between laboratories, most notably the presence/absence of ledges at the sides of open arms and the transparency/opaqueness of closed arm walls. In a previous report, we used a custom designed container to compare rat behavior on different combinations of open and closed arm designs under otherwise identical experimental conditions, and showed that differences in arm features interfere with experimental outcomes. In brief, open arm ledges significantly increased anxiety-like behavior in rats, while transparent arms may also have decreased this behavior. Furthermore, we verified a higher detection sensitivity of the effect of an anxiolytic drug in a combination of no-ledged open arm + opaque walled closed arm compared with a combination of ledged open arm + transparent walled closed arm. In this report, we introduce our protocol for the elevated plus maze test, together with discussion of the key results from the previous report and our experimental experience. We believe this report will provide useful information for researchers who have employed or who plan to use the elevated plus maze in their studies.

Rats' anxiety-like behavior tested by the elevated plus maze varies highly depending on the testing environment. Here we show how apparatus settings, especially arm structure, affect behavior on the maze, and we explain a reliable protocol to detect effects of anxiolytic drugs. This report will contribute to anxiety research.

大鼠动物焦虑症的实验研究: 开放臂壁和闭合臂墙透明度对高加迷宫试验的影响

The elevated plus maze test is a behavioral test for assessing animal anxiety in rodents. Although this test is widely applied in the field of behavioral ...

Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms

The Barnes maze is a reliable measure of spatial learning and memory that does not require food restriction or exposure to extremely stressful stimuli. The Barnes maze can also assess other mouse behaviors, such as general motivation to escape from the maze platform and exploratory behavior. The Barnes maze can measure whether a genetic mutation or environmental variable can impact the acquisition and retention of spatial memories, as well as provide information about the search strategy employed by the mice. Here we use the Barnes maze to detect a memory deficit in adult mice following a single developmental ethanol exposure event. The newly described Damsel-in-Distress paradigm exposes a male mouse to a female mouse trapped in a chamber in the open center field of the arena. It provides an opportunity for the mouse to socially respond to the trapped female and exhibit prosocial behavior. The Damsel-in-Distress paradigm can also be used to examine mouse behavior in a novel arena and measure locomotor activity. Both the Barnes Maze and the Damsel-in-Distress protocols require minimal financial investment and most aspects of the tests can be constructed from common lab supplies. These flexible and accessible tools can also be used to detect behavioral changes over the course of development.

This protocol measures spatial learning and memory using the Barnes maze. A novel Damsel-in-Distress paradigm is used to assess locomotor activity and prosocial behavior in mice.

利用巴恩斯迷宫和困境中的破坏范式分析小鼠的空间学习和社会行为

The Barnes maze is a reliable measure of spatial learning and memory that does not require food restriction or exposure to extremely stressful stimuli. ...