Name: in vivo, percutaneous, needle based, optical coherence tomography of renal masses
Uploaded: 2015-03-30T14:00:00.000+00:00
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Description: Watch this Scientific Journal Video about In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses at JoVE.com

This work is funded by the Cure for Cancer Foundation, Dutch Technology Foundation (STW) and The Netherlands Organisation for Health Research and Development (ZonMw).

所提出的过程需要根据批准的学术医学中心的阿姆斯特丹，注册号NL41985.018的机构审查委员会研究方案的地方。书面知情同意从所有参与者必需的。 1.系统<ol><li>对于这个实验，使用傅立叶域OCT系统，在1,280-1,350纳米的波长带13运行。傅立叶域低相干干涉测量法允许连续扫描时相比第一代时域OCT系统，这增加了数据采集速度。注意:OCT系统在〜90°角接口与光纤探针，扫描螺旋形。它具有2.7F的外径（0.9毫米）和135厘米插入长度。探头连接到OCT控制台通过一个驱动电机和光学控制器（安装底座）为54毫米的范围内拉回。所获取的十月数据集包括541横截面图像（B扫描）瓦特第i个15微米的轴向分辨率（ 图1，2）。 </li><li>为了保证准确和可重复的衰减测量，通过测量校准μ 十月用于增加基于脂肪乳剂由Kodach 等人 14,15先前所描述的重量百分比，（ 例如脂肪乳）的浓度。 简而言之: <ol><li>稀释标准批量的20％脂肪乳剂用软化水的H 2 O来实现的0.125，0.250，0.5，1.0，2.0，4.0，10，15和20（股票）百分浓度。 <ol><li>放置在200毫升的脂肪乳剂的混合物在OCT探针和取得的OCT测量。 </li><li>交叉引用提取μ 十月与文献已知值的值。 </li></ol></li></ol></li></ol> 2.超时和患者定位<ol><li>在此之前启动的程序，执行"超时"检查姓名，出生，程序，PR日期ocedural侧，抗凝剂使用，和过敏。 </li><li>取决于肿瘤的位置，放置在患者中任俯卧或侧卧位。为患者提供足够的支持和验证，如果他/她希望能安心在这个位置在20到40分钟内。 </li><li>使用超声（美国）16，定位肿瘤，并标记与永久性墨水皮肤针切入点。 注:使用计算机断层扫描（CT），使用灵活的针指导模板本地化接入针的首选位置。 </li></ol> 3.消毒和无菌悬垂<ol><li>换上了手术帽口盖。 </li><li>清洗使用洗必泰/醇溶液围绕穿刺部位的皮肤上，注意不要取出先前放置针条目标记（步骤2.3）。消毒广域将防止的必要性的情况下意外访问针重新定位额外的清洁。 </li><li>其中r无菌内容egard，打开经皮穿刺组包含:10毫升注射器，钝抽吸针，21g的注射针，手术刀，一个15政同轴导引针，一个18G的套管针，和一个16摹核心活检枪。 </li><li>彻底洗净双手，将手消毒之后。换上了手术服和无菌手套。 </li><li>覆盖患者在无菌窗帘。 </li><li>应用无菌罩周围的超声探头和修复到位的导针。 </li></ol> 4.准备华侨城<ol><li>启动OCT控制台，然后输入使用控制台界面标记患者ID字段，姓氏，名字和出生日期（出生日期）病人的详细信息。 </li><li>用无菌的内容方面，解压缩包含的OCT探针，无菌的安装基座盖，和5ml的路厄锁注射器在OCT封装。 </li><li>适用于无菌盖华侨城控制台安装基座。引导非无菌安装基座需要的在助手的帮助。 </li><li>填写5毫升注射器用0.9％氯化钠并将其连接到冲洗端口。冲洗OCT探针直到水出现在探针盖的远端部分。 </li><li>加载华侨城探头插入安装底座。后装载探针将旋转并发射红光确认适当运作。离开探头在其保护盖冲洗和加载减少损失的风险中。 </li><li>从盖取下OCT探头。放置在坚硬的表面上的探针和使用手术刀缩短小费。切割，以尽量减少在光纤和棱镜在压力期间固定所述探针的远端部分。切断从棱镜5毫米远端，使用发射（红色）光的方向。 </li></ol> 5.穿刺<ol><li>麻醉皮肤和用2％利多卡因（20毫克/毫升）深的层。等待几分钟，允许利多卡因生效。询问患者是否有任何痛苦。 </li><li>使用针导向，放置为15G同轴引导针验证通过成像的位置。如果位置是令人满意的，除去闭孔（尖锐的针芯）。 </li><li>将18G的套管针通过引导针，刺入肿瘤。再次验证与成像针的位置。如果位置是令人满意的去除闭孔。 </li><li>饲料华侨城探头向上套管针，直到感觉阻力。 </li><li>同时固定在OCT探头，缩回套管针，露出在OCT探头至肿瘤组织。保持肿瘤内套针针尖时呼吸周期最小化在OCT探针的扭结。这降低了探头损坏的风险。 </li><li> OCT扫描: <ol><li>执行十月扫描，与控制台定为每541集B-扫描。这里使用的OCT系统将超过5.4厘米无需特定参数的调整的长度执行自动回调。 </li><li>检查的扫描质量，文物和实体组织的外观（图1a）。伪影最常出现圆形条带从正常十月图案（ 图1B）中脱颖而出。 </li><li>更换探头如果重新扫描后的文物仍然存在。 </li></ol></li><li>重复步骤5.6，直到至少10月3日的数据集被获取。 </li><li>除去在OCT探针和套管针，而使导引针就地。 </li><li>武装核心活检枪，并把它通过引导针，确认上成像的位置。 </li><li>如果定位是令人满意的，火活检枪。 </li><li>根据病理科的协议将活检材料的容器中。这里，地点活检上的培养皿用纸镶嵌，充分饱和的0.9％NaCl。 </li><li>检查活检的质量和，直到获得足够的材料重复步骤5.9和5.10。 </li></ol>

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The authors of this article have nothing to disclose.

在本出版物中，我们报告了经皮，针根据肾，华侨城的可行性。这是华侨城发展的至关重要的第一步成肿瘤的分化临床应用技术，称为"光活检"。我们的第25例患者表现出经皮华侨城是一个简单而安全的过程。光学活检有两个好处比传统的核心活检。首先，将实时采集的OCT数据和分析将提供即时诊断的结果，相对于5-10天的常规病理学的处理时间。第二，OCT具有减​​少的非诊断的程序，这是常规?...

在过去几十年已经表明肾群众1,2的发病率稳步上升。到现在为止，肾传治疗决策已经作出主要根据MRI和CT成像特性，年龄和合并症的基础。然而，这些诊断方法和临床参数缺乏技巧，真正检测肾质量的恶性潜能。核心活检或细针穿刺有足够的组织进行病理评估（诊断）提供了客观的肿瘤分化程度与敏感性和特异性在95-100％3的范围内。因此活检获得接受可疑肾群众4,5的评价。然而，活组织检查没有足够的组织建立了诊断或具有在10-20％的速度正常肾实质（非诊断）发生整体，甚至高达30％的在肾脏小块（&lt;4厘米，的SRM），延迟由于频繁必要性额外诊断过程活检手术3,5。 光学相干断层扫描（OCT）是一种新型的成像模态具有克服在肾传分化上述障碍的可能性。根据近红外光的散射，十月提供了一个15微米的轴向分辨率的图像在2-3毫米的有效组织渗透（ 图1，2）。每组织渗透，组织特异性光散射的所得中，毫米波信号强度的损失被表示为衰减系数（μ 十月 :毫米-1）所描述的费伯等人6。组织学特征可以与可μ 十月值提供用于组织的分化（ 图3）的定量参数。 期间发生，恶性细胞显示增加的数目，以及更大和更不规则形状的核<html>更高的折射率，更积极的线粒体。由于这种过表达细胞成分，在μ 十月的变化是比较恶性肿瘤良性肿瘤或未受影响的组织7时可以预期的。 最近我们研究了浅表华侨城良性和恶性肾群众8,9区分的能力。在16名患者，使用外部放置十月探针获得肿瘤组织的手术中十月测量。该控制臂由未受影响的组织中的同一患者的OCT测量。正常组织呈显著较低位数衰减系数相比恶性组织，证实OCT的肿瘤分化的潜力。这种定量分析是在一个类似的方式向分级其他类型的恶性组织中，被施加如尿路上皮癌10,11和外阴上皮瘤分化12。 ENT"&gt;我们的目标是发展成为十月的光学活检，提供实时影像结合在现场肿瘤分化。目前研究的目的是来形容一个经皮，针基础，华侨城患者的方法诊断为固体增强肾质量，这种方法的描述是，就我们所知，在第一评估针的肾肿瘤的可能性基于十月。

<table><tbody><tr><td>15 G/7.5 cm Co-Axial Introducer Needle</td><td>Angiotech, Gainesville, USA</td><td>MCXS1612SX</td><td></td></tr><tr><td>18 G/20 cm Trocar Needle</td><td>Cook medical, Bloomington, USA</td><td>DTN-18-20.0-U</td><td></td></tr><tr><td>16 G/20 cm Quick-Core Biopsy Gun</td><td>Cook Medical, Bloomington, USA</td><td>G07827</td><td></td></tr><tr><td>Ilumien Optis PCI Optimization System (OCT &amp;amp; FFR)</td><td>St. Jude medical, St. Paul, USA</td><td>C408650</td><td>Part of Dragonfly Kit.&lt;br/&gt; St. Jude medical, St. Paul, USA. (C4088643)</td></tr><tr><td>Dragonfly Duo Imaging Catheter</td><td>LightLab Imaging, Westford, USA</td><td>C408644</td><td>Part of Dragonfly Kit.&lt;br/&gt; St. Jude medical, St. Paul, USA. (C4088643)</td></tr><tr><td>Sterile Dock Cover</td><td>CFI Med. Solutions, Fenton, USA</td><td>200-700-00</td><td>Part of Dragonfly Kit.&lt;br/&gt; St. Jude medical, St. Paul, USA. (C4088643)</td></tr><tr><td>5 ml Luer-lock Syringe</td><td>Merit Med. Syst., South Jordan, USA</td><td>C408647</td><td></td></tr><tr><td>10 ml Syringe</td><td>BD, Franklin Lakes, USA</td><td>300912</td><td></td></tr><tr><td>18 G Blunt Fill Needle</td><td>BD, Franklin Lakes, USA</td><td>305180</td><td></td></tr><tr><td>21 G Injection Needle</td><td>BD, Franklin Lakes, USA</td><td>301155</td><td></td></tr><tr><td>Sterile scalpel</td><td>BD, Franklin Lakes, USA</td><td>372611</td><td></td></tr><tr><td>NaCl 0,9% solution</td><td>Braun, Melsungen AG, Germany</td><td>222434</td><td></td></tr><tr><td>Lidoca&amp;iuml;ne HCl 2% (20 mg/ml) solution</td><td>Braun, Melsungen AG, Germany</td><td>3624480</td><td></td></tr><tr><td>Sterile Ultrasound Gel, Aquasonic 100</td><td>Parker Lab. Inc., Fairfield, USA</td><td>GE424609</td><td></td></tr><tr><td>Sterile Ultrasound Cover</td><td>Microtek Med., Alpharetta, USA</td><td>PC1289EU</td><td></td></tr><tr><td>Pathology Container</td><td></td><td></td><td></td></tr><tr><td>AMIRA software package</td><td>FEI Visualization Sciences Group, Hillsboro, USA</td><td>Software platform for 3D data analysis</td><td></td></tr><tr><td>FIJI software package (open source)</td><td>Open source, http://fiji.sc/Fiji</td><td></td><td>Open source image processing software</td></tr></tbody></table>

in vivo, percutaneous, needle based, optical coherence tomography of renal masses

Optical coherence tomography (OCT) is the optical equivalent of ultrasound imaging, based on the backscattering of near infrared light. OCT provides real time images with a 15 &#181;m axial resolution at an effective tissue penetration of 2-3 mm. Within the OCT images the loss of signal intensity per millimeter of tissue penetration, the attenuation coefficient, is calculated. The attenuation coefficient is a tissue specific property, providing a quantitative parameter for tissue differentiation. 
Until now, renal mass treatment decisions have been made primarily on the basis of MRI and CT imaging characteristics, age and comorbidity. However these parameters and diagnostic methods lack the finesse to truly detect the malignant potential of a renal mass. A successful core biopsy or fine needle aspiration provides objective tumor differentiation with both sensitivity and specificity in the range of 95-100%. However, a non-diagnostic rate of 10-20% overall, and even up to 30% in SRMs, is to be expected, delaying the diagnostic process due to the frequent necessity for additional biopsy procedures. 
We aim to develop OCT into an optical biopsy, providing real-time imaging combined with on-the-spot tumor differentiation. This publication provides a detailed step-by-step approach for percutaneous, needle based, OCT of renal masses.

中的第一25肿瘤（23例），一共有24个成功的OCT程序进行。在一种情况下，一个探测器故障导致无法获得的OCT扫描。两个不良事件（AE）发生，其中详细讨论部分中描述。一般患者的特征见于表1。 华侨城控制台已预装软件获得的数据集的直接定性分析提供实时OCT图像。进一步分析和衰减测量，华侨城的数据可以导出为原始数据，TIFF，DICOM和AVI格式。 μ的华侨城<...

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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal masses.

在体内 ，经皮，基于针，肾群众的光学相干断层扫描

Optical coherence tomography (OCT) is the optical equivalent of ultrasound imaging, based on the backscattering of near infrared light. OCT provides real ...

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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

8.8K 次观看.  Academic Medical Center. Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal masses.

视频: In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

Lung cancer is the leading cause of cancer-related deaths1. Squamous cell and small cell cancers typically arise in association with the conducting airways, whereas adenocarcinomas are typically more peripheral in location. Lung malignancy detection early in the disease process may be difficult due to several limitations: radiological resolution, bronchoscopic limitations in evaluating tissue underlying the airway mucosa and identifying early pathologic changes, and small sample size and/or incomplete sampling in histology biopsies. High resolution imaging modalities, such as optical frequency domain imaging (OFDI), provide non-destructive, large area 3-dimensional views of tissue microstructure to depths approaching 2 mm in real time (Figure 1)2-6. OFDI has been utilized in a variety of applications, including evaluation of coronary artery atherosclerosis6,7 and esophageal intestinal metaplasia and dysplasia6,8-10.
Bronchoscopic OCT/OFDI has been demonstrated as a safe in vivo imaging tool for evaluating the pulmonary airways11-23 (Animation). OCT has been assessed in pulmonary airways16,23 and parenchyma17,22 of animal models and in vivo human airway14,15. OCT imaging of normal airway has demonstrated visualization of airway layering and alveolar attachments, and evaluation of dysplastic lesions has been found useful in distinguishing grades of dysplasia in the bronchial mucosa11,12,20,21. OFDI imaging of bronchial mucosa has been demonstrated in a short bronchial segment (0.8 cm)18. Additionally, volumetric OFDI spanning multiple airway generations in swine and human pulmonary airways in vivo has been described19. Endobronchial OCT/OFDI is typically performed using thin, flexible catheters, which are compatible with standard bronchoscopic access ports. Additionally, OCT and OFDI needle-based probes have recently been developed, which may be used to image regions of the lung beyond the airway wall or pleural surface17.
While OCT/OFDI has been utilized and demonstrated as feasible for in vivo pulmonary imaging, no studies with precisely matched one-to-one OFDI:histology have been performed. Therefore, specific imaging criteria for various pulmonary pathologies have yet to be developed. Histopathological counterparts obtained in vivo consist of only small biopsy fragments, which are difficult to correlate with large OFDI datasets. Additionally, they do not provide the comprehensive histology needed for registration with large volume OFDI. As a result, specific imaging features of pulmonary pathology cannot be developed in the in vivo setting. Precisely matched, one-to-one OFDI and histology correlation is vital to accurately evaluate features seen in OFDI against histology as a gold standard in order to derive specific image interpretation criteria for pulmonary neoplasms and other pulmonary pathologies. Once specific imaging criteria have been developed and validated ex vivo with matched one-to-one histology, the criteria may then be applied to in vivo imaging studies. Here, we present a method for precise, one to one correlation between high resolution optical imaging and histology in ex vivo lung resection specimens. Throughout this manuscript, we describe the techniques used to match OFDI images to histology. However, this method is not specific to OFDI and can be used to obtain histology-registered images for any optical imaging technique. We performed airway centered OFDI with a specialized custom built bronchoscopic 2.4 French (0.8 mm diameter) catheter. Tissue samples were marked with tissue dye, visible in both OFDI and histology. Careful orientation procedures were used to precisely correlate imaging and histological sampling locations. The techniques outlined in this manuscript were used to conduct the first demonstration of volumetric OFDI with precise correlation to tissue-based diagnosis for evaluating pulmonary pathology24. This straightforward, effective technique may be extended to other tissue types to provide precise imaging to histology correlation needed to determine fine imaging features of both normal and diseased tissues.

Optical Frequency Domain Imaging of Ex vivo Pulmonary Resection Specimens: Obtaining One to One Image to Histopathology Correlation

A method to image ex vivo pulmonary resection specimens with optical frequency domain imaging (OFDI) and obtain precise correlation to histology is described, which is essential to developing specific OFDI interpretation criteria for pulmonary pathology. This method is applicable to other tissue types and imaging techniques to obtain precise imaging to histology correlation for accurate image interpretation and assessment. Imaging criteria established with this technique would then be applicable to image assessment in future in vivo studies.

光频域成像<em&gt;体外</em肺切除标本：获得一对一的图像病理组织学的相关性

Lung cancer is the leading cause of cancer-related deaths1. Squamous cell and small cell cancers typically arise in association with the conducting ...

科研

生物工程

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Tumor spheroids have been developed as a three-dimensional (3D) cell culture model in cancer research and anti-cancer drug discovery. However, currently, high-throughput imaging modalities utilizing bright field or fluorescence detection, are unable to resolve the overall 3D structure of the tumor spheroid due to limited light penetration, diffusion of fluorescent dyes and depth-resolvability. Recently, our lab demonstrated the use of optical coherence tomography (OCT), a label-free and non-destructive 3D imaging modality, to perform longitudinal characterization of multicellular tumor spheroids in a 96-well plate. OCT was capable of obtaining 3D morphological and physiological information of tumor spheroids growing up to about 600 &#181;m in height. In this article, we demonstrate a high-throughput OCT (HT-OCT) imaging system that scans the whole multi-well plate and obtains 3D OCT data of tumor spheroids automatically. We describe the details of the HT-OCT system and construction guidelines in the protocol. From the 3D OCT data, one can visualize the overall structure of the spheroid with 3D rendered and orthogonal slices, characterize the longitudinal growth curve of the tumor spheroid based on the morphological information of size and volume, and monitor the growth of the dead-cell regions in the tumor spheroid based on optical intrinsic attenuation contrast. We show that HT-OCT can be used as a high-throughput imaging modality for drug screening as well as characterizing biofabricated samples.

Optical coherence tomography (OCT), a three-dimensional imaging technology, was used to monitor and characterize the growth kinetics of multicellular tumor spheroids. Precise volumetric quantification of tumor spheroids using a voxel counting approach, and label-free dead tissue detection in the spheroids based on intrinsic optical attenuation contrast, were demonstrated.

三维肿瘤球体的纵向形态和生理监测

Tumor spheroids have been developed as a three-dimensional (3D) cell culture model in cancer research and anti-cancer drug discovery. However, currently, ...

癌症研究

使用眼前节 HR-OCT 评估眼表鳞状瘤变

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia

Ocular surface squamous neoplasia (OSSN) is the most common tumor of the ocular surface, ranging from mild dysplasia to invasive squamous carcinoma. Traditionally, the diagnosis of OSSN relies on histopathological confirmation followed by a full-thickness biopsy. However, in the past two decades, the therapeutic approach to OSSN has shifted from surgical intervention to topical chemotherapy regimens in clinical settings. This shift emphasizes the need for less invasive or non-invasive methods to diagnose ocular surface pathologies. Among various imaging devices, commercially available high-resolution optical coherence tomography (HR-OCT) has emerged as a powerful tool for the characterization of OSSN. HR-OCT provides an in vivo, cross-sectional view of ocular surface lesions, offering an "optical biopsy" for OSSN with high sensitivity and specificity. It provides valuable information in differentiating intraepithelial or invasive OSSN from other benign lesions. Additionally, HR-OCT can be used to monitor the response to topical chemotherapy and to detect subclinical OSSN during follow-up visits. In this article, the scanning protocol for image acquisition is presented, and image interpretation for OSSN is outlined. This standardized, practical, and reproducible approach is recommended in clinical workflows and is expected to assist clinicians in the management of OSSN.

作者聚焦： 前部 HR-OCT 作为表征眼表鳞状瘤变的非侵入性工具

Anterior segment high-resolution optical coherence tomography (HR-OCT) is a promising non-invasive modality for the diagnosis and therapeutic evaluation of ocular surface squamous neoplasia (OSSN). Here, the system setup, scanning technique, and representative diagnostic results are presented.

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

Ocular surface squamous neoplasia (OSSN) is the most common tumor of the ocular surface, ranging from mild dysplasia to invasive squamous carcinoma. ...

工程学

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Structural changes in the retina are common manifestations of ophthalmic diseases. Optical coherence tomography (OCT) enables their identification in vivo&#8212;rapidly, repetitively, and at a high resolution. This protocol describes OCT imaging in the mouse retina as a powerful tool to study optic neuropathies (OPN). The OCT system is an interferometry-based, non-invasive alternative to common post mortem histological assays. It provides a fast and accurate assessment of retinal thickness, allowing the possibility to track changes, such as retinal thinning or thickening. We present the imaging process and analysis with the example of the Opa1delTTAG mouse line. Three types of scans are proposed, with two quantification methods: standard and homemade calipers. The latter is best for use on the peripapillary retina during radial scans; being more precise, is preferable for analyzing thinner structures. All approaches described here are designed for retinal ganglion cells (RGC) but are easily adaptable to other cell populations. In conclusion, OCT is efficient in mouse model phenotyping and has the potential to be used for the reliable evaluation of therapeutic interventions.

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

This manuscript describes a protocol for the in vivo imaging of the mouse retina with high-resolution spectral domain optical coherence tomography (SD-OCT). It focuses on retinal ganglion cells (RGC) in the peripapillary region, with several scanning and quantifying approaches described.

光学相干断层扫描: 成像小鼠视网膜神经节细胞在体内

Structural changes in the retina are common manifestations of ophthalmic diseases. Optical coherence tomography (OCT) enables their identification in ...

神经科学

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model

Laser-induced choroidal neovascularization (CNV) is a well-established model to mimic the wet form of age-related macular degeneration (AMD). In this protocol, we aim to guide the reader not simply through the technical considerations of generating laser-induced lesions to trigger neovascular processes, but rather focus on the powerful information that can be obtained from multimodal longitudinal in vivo imaging throughout the follow-up period.
The laser-induced mouse CNV model was generated by a diode laser administration. Multimodal in vivo imaging techniques were used to monitor CNV induction, progression and regression. First, spectral domain optical coherence tomography (SD-OCT) was performed immediately after the lasering to verify a break of Bruch&#39;s membrane. Subsequent in vivo imaging using fluorescein angiography (FA) confirmed successful damage of Bruch&#39;s membrane from serial images acquired at the choroidal level. Longitudinal follow-up of CNV proliferation and regression on days 5, 10, and 14 after the lasering was performed using both SD-OCT and FA. Simple and reliable grading of leaky CNV leasions from FA images is&#160;presented. Automated segmentation for measurement of total retinal thickness, combined with manual caliber application for measurement of retinal thickness at CNV sites, allow unbiased evaluation of the presence of edema. Finally, histological verification of CNV is performed using isolectin GS-IB4 staining on choroidal flatmounts. The staining is thresholded, and the isolectin-positive area is calculated with ImageJ.
This protocol is especially useful in therapeutics studies requiring high-throughput-like screening of CNV pathology as it allows fast, multimodal, and reliable classification of CNV pathology and retinal edema. In addition, high resolution SD-OCT enables the recording of other pathological hallmarks, such as the accumulation of subretinal or intraretinal fluid. However, this method does not provide a possibility to automate CNV volume analysis from SD-OCT images, which has to be performed manually.

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model

Here, we present the usefulness of longitudinal in vivo imaging in the follow-up of morphological changes of laser-induced choroidal neovascularization in mice.

在体内小鼠激光诱导脉络膜新生血管模型的多模态成像与分析

Laser-induced choroidal neovascularization (CNV) is a well-established model to mimic the wet form of age-related macular degeneration (AMD). In this ...

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Spectral-domain optical coherence tomography (SD-OCT) is useful for visualizing retinal and ocular structures in vivo. In research, SD-OCT is a valuable tool to evaluate and characterize changes in a variety of retinal and ocular disease and injury models. In light induced retinal degeneration models, SD-OCT can be used to track thinning of the photoreceptor layer over time. In glaucoma models, SD-OCT can be used to monitor decreased retinal nerve fiber layer and total retinal thickness and to observe optic nerve cupping after inducing ocular hypertension. In diabetic rodents, SD-OCT has helped researchers observe decreased total retinal thickness as well as decreased thickness of specific retinal layers, particularly the retinal nerve fiber layer with disease progression. In mouse models of myopia, SD-OCT can be used to evaluate axial parameters, such as axial length changes. Advantages of SD-OCT include in vivo imaging of ocular structures, the ability to quantitatively track changes in ocular dimensions over time, and its rapid scanning speed and high resolution. Here, we detail the methods of SD-OCT and show examples of its use in our laboratory in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia. Methods include anesthesia, SD-OCT imaging, and processing of the images for thickness measurements.

Here, we describe the use of spectral-domain optical coherence tomography (SD-OCT) to visualize retinal and ocular structures in vivo in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia.

使用光学相干断层扫描对啮齿动物模型中眼部疾病的体内结构评估

Spectral-domain optical coherence tomography (SD-OCT) is useful for visualizing retinal and ocular structures in vivo. In research, SD-OCT is a valuable ...

Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography

The vascular supply to the retina has been shown to dynamically adapt through vasoconstriction and vasodilation to accommodate the metabolic demands of the retina. This process, referred to as retinal vascular reactivity (RVR), is mediated by neurovascular coupling, which is impaired very early in retinal vascular diseases such as diabetic retinopathy. Therefore, a clinically feasible method of assessing vascular function may be of significant interest in both research and clinical settings. Recently, in vivo imaging of the retinal vasculature at the capillary level has been made possible by the FDA approval of optical coherence tomography angiography (OCTA), a noninvasive, minimal risk and dyeless angiography method with capillary level resolution. Concurrently, physiological and pathological changes in RVR have been shown by several investigators. The method shown in this manuscript is designed to investigate RVR using OCTA with no need for alterations to the clinical imaging procedures or device. It demonstrates real time imaging of the retina and retinal vasculature during exposure to hypercapnic or hyperoxic conditions. The exam is easily performed with two personnel in under 30 min with minimal subject discomfort or risk. This method is adaptable to other ophthalmic imaging devices and the applications may vary based on the composition of the gas mixture and patient population. A strength of this method is that it allows for an investigation of retinal vascular function at the capillary level in human subjects in vivo. Limitations of this method are largely those of OCTA and other retinal imaging methods including imaging artifacts and a restricted dynamic range. The results obtained from the method are OCT and OCTA images of the retina. These images are amenable to any analysis that is possible on commercially available OCT or OCTA devices. The general method, however, can be adapted to any form of ophthalmic imaging.

This article describes a method for measuring retinal vasculature reactivity in vivo with human subjects using a gas breathing provocation technique to deliver vasoactive stimuli while acquiring retinal images.

光相一致性断层扫描血管造影评估的视网膜血管反应

The vascular supply to the retina has been shown to dynamically adapt through vasoconstriction and vasodilation to accommodate the metabolic demands of ...

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Optical coherence tomography (OCT) offers a noninvasive method for the diagnosis of retinopathy. The OCT machine can capture retinal crosssectional images from which the retinal thickness can be calculated. Although OCT is widely used in clinical practice, its application in basic research is not as prevalent, especially in small animals such as mice. Because of the small size of their eyeballs, it is challenging to conduct fundus imaging examinations in mice. Therefore, a specialized retinal imaging system is required to accommodate OCT imaging on small animals. This article demonstrates a small-animal-specific system for OCT examination procedures and a detailed method for image analysis. The results of retinal OCT examination of very-low-density lipoprotein receptor (Vldlr) knockout mice and C57BL/6J mice are presented. The OCT images of C57BL/6J mice showed retinal layers, while those of Vldlr knockout mice showed subretinal neovascularization and retinal thinning. In summary, OCT examination could facilitate the noninvasive detection and measurement of retinopathy in mouse models.

Here, we describe an in vivo imaging technique using optical coherence tomography to facilitate the diagnosis and quantitative measurement of retinopathy in mice.

光学相干断层扫描在视网膜病变小鼠模型中的应用

Optical coherence tomography (OCT) offers a noninvasive method for the diagnosis of retinopathy. The OCT machine can capture retinal crosssectional images ...

在体内，经皮，基于针，肾群众的光学相干断层扫描

摘要

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此视频中的章节

光频域成像

三维肿瘤球体的纵向形态和生理监测

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

光学相干断层扫描: 成像小鼠视网膜神经节细胞在体内

在体内小鼠激光诱导脉络膜新生血管模型的多模态成像与分析

使用光学相干断层扫描对啮齿动物模型中眼部疾病的体内结构评估

光相一致性断层扫描血管造影评估的视网膜血管反应

光学相干断层扫描在视网膜病变小鼠模型中的应用

在体内 ，经皮，基于针，肾群众的光学相干断层扫描

摘要

探索更多视频

此视频中的章节

光频域成像

三维肿瘤球体的纵向形态和生理监测

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

前路高分辨率光学相干断层扫描在眼表鳞状瘤变诊断和治疗监测中的应用

光学相干断层扫描: 成像小鼠视网膜神经节细胞在体内

在体内小鼠激光诱导脉络膜新生血管模型的多模态成像与分析

使用光学相干断层扫描对啮齿动物模型中眼部疾病的体内结构评估

光相一致性断层扫描血管造影评估的视网膜血管反应

光学相干断层扫描在视网膜病变小鼠模型中的应用

在体内，经皮，基于针，肾群众的光学相干断层扫描