Generation of Primary Mammospheres from Human Breast Cancer Cell Lines
4:54
Generation of Primary Mammospheres from Human Breast Cancer Clinical Samples
7:04
Mammosphere Forming Efficiency (%) Calculation
7:42
Serial Passaging of Mammospheres for Assessment of Self-renewal
9:18
Results: Estimation of Sphere Forming Efficiancy in MCF7 and MDA-231 Cells
10:04
Conclusion
Floating mammosphere assays can investigate the subset of stem-like breast cancer cells that survive in suspension conditions and show enhanced tumorigenesis when implanted into mice. This protocol provides a convenient in vitro measure of sphere-forming ability, a proxy for in vivo tumorigenesis, while facilitating analysis of the stem-associated transcriptional landscape.
