Synthesis of Protein-templated Gold Nanoclusters (Au NCs)
3:17
Screen Relative Binding Affinity of Different Small Molecular Drugs to HSA
5:19
Measure the Binding Constant of a Specific Drug to HSA
6:41
Results: Studying Protein-drug Binding by Forming Fluorescent Gold Nanoclusters (Au NCs) using Drug-loaded Serum Albumen as Template
7:53
Conclusion
A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.
