Labeling of the Reducing End (RE) Sugar Residue of W-sol AXs with 2-aminobenzamide (2AB)
2:11
Generation of Xylo-oligosaccharides from 2 AB Labeled W-sol AXs
3:34
Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF MS)
4:48
Electrospray Ionization Quadrupole Time-of-flight Mass Spectrometry (ESI-QTOF MS)
6:20
ESI-MSn
7:30
H1 Nuclear Magnetic Resonance (NMR) Spectroscopy
8:45
Results: Reducing End (RE) and Internal Region Glycosyl Sequence(s) of Heteroxylans
10:34
Conclusion
副本
The overall goal is to characterize the reducing end and internal region glycosal sequences of heteroxylans by tagging the original reducing end sugar residue of the water-soluble heravenaxylans and treating with an endoxylanase to generate a mixt
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This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).