Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

7.6K Views

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11:49 min

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March 24th, 2016

DOI :

10.3791/53748-v

March 24th, 2016

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Sunil Ratnayake¹, Kristina Ford¹, Antony Bacic¹

¹ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, University of Melbourne

Summary

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Keywords Plant Wall Heteroxylans

Reducing End

Internal Region

Glycosal Sequences

Water soluble Heteroxylans

Polysaccharide Sequencing

Chapters in this video

0:05

Title

0:53

Labeling of the Reducing End (RE) Sugar Residue of W-sol AXs with 2-aminobenzamide (2AB)

2:11

Generation of Xylo-oligosaccharides from 2 AB Labeled W-sol AXs

3:34

Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF MS)

4:48

Electrospray Ionization Quadrupole Time-of-flight Mass Spectrometry (ESI-QTOF MS)

6:20

ESI-MSⁿ

7:30

H¹ Nuclear Magnetic Resonance (NMR) Spectroscopy

8:45

Results: Reducing End (RE) and Internal Region Glycosyl Sequence(s) of Heteroxylans

10:34

Conclusion

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