The overall goal of the protocol is the production and purification of human norovirus protruding domain in E.coli in order to obtain extremely pure protein for X-ray crystallography. Our protocol for protein expression, purification, and crystall
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Here, we describe a method to express and purify high quality norovirus protruding (P) domains in E. coli for use in X-ray crystallography studies. This method can be applied to other calicivirus P domains, as well as non-structural proteins, i.e., viral protein genome-linked (VPg), protease, and RNA dependent RNA polymerase (RdRp).
