Results: Aphid Ovary Dissection and Whole-mount Immunostaining with Aphid-specific Conditions to Increase Tissue Permeability and Decrease Background Staining
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Conclusion
副本
The overall goal of this immunostaining protocol is to detect protein expression in the aphid embryos. Critical conditions are identified that increase tissue permeability and decrease background staining, both of which are long-standing problems
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A protocol of whole-mount immunostaining on aphid embryos is presented, in which critical conditions for decreasing background staining and increasing tissue permeability are addressed. These conditions are specifically developed for effective detection of protein expression in the embryonic tissues of aphids.